Effect of Low Versus High Tidal-Volume Total Liquid Ventilation on Pulmonary Inflammation

Michaël Sage; Wendy See; Stéphanie Nault; Christophe Morin; Christina Michalski; Benoit Chabot; Sofia Marouan; Pascal M Lavoie; Philippe Micheau; Jean-Paul Praud; Étienne Fortin-Pellerin

doi:10.3389/fphys.2020.00603

Effect of Low Versus High Tidal-Volume Total Liquid Ventilation on Pulmonary Inflammation

Front Physiol. 2020 Jun 18:11:603. doi: 10.3389/fphys.2020.00603. eCollection 2020.

Authors

Michaël Sage¹, Wendy See¹, Stéphanie Nault¹, Christophe Morin¹, Christina Michalski^{2

3}, Benoit Chabot⁴, Sofia Marouan⁵, Pascal M Lavoie^{2

3

6}, Philippe Micheau⁷, Jean-Paul Praud^{1

8}, Étienne Fortin-Pellerin^{1

8}

Affiliations

¹ Department of Pharmacology-Physiology, Université de Sherbrooke, Sherbrooke, QC, Canada.
² BC Children's Hospital Research Institute, The University of British Columbia, Vancouver, BC, Canada.
³ Department of Experimental Medicine, The University of British Columbia, Vancouver, BC, Canada.
⁴ Department of Microbiology and Infectiology, Université de Sherbrooke, Sherbrooke, QC, Canada.
⁵ Department of Pathology, Université de Sherbrooke, Sherbrooke, QC, Canada.
⁶ Department of Pediatrics, The University of British Columbia, Vancouver, BC, Canada.
⁷ Department of Mechanical Engineering, Université de Sherbrooke, Sherbrooke, QC, Canada.
⁸ Department of Pediatrics, Université de Sherbrooke, Sherbrooke, QC, Canada.

Abstract

Animal experiments suggest that total liquid ventilation (TLV) induces less ventilator-induced lung injury (VILI) than conventional mechanical gas ventilation. However, TLV parameters that optimally minimize VILI in newborns remain unknown. Our objective was to compare lung inflammation between low (L-V_T) and high (H-V_T) liquid tidal volume and evaluate impacts on the weaning process. Sixteen anesthetized and paralyzed newborn lambs were randomized in an L-V_T group (initial tidal volume of 10 mL/kg at 10/min) and an H-V_T group (initial tidal volume of 20 mL/kg at 5/min). Five unventilated newborn lambs served as controls. After 4 h of TLV in the supine position, the lambs were weaned in the prone position for another 4 h. The levels of respiratory support needed during the 4 h post-TLV were compared. The anterior and posterior lung regions were assessed by a histological score and real-time quantitative PCR for IL1B, IL6, and TNF plus 12 other exploratory VILI-associated genes. All but one lamb were successfully extubated within 2 h post-TLV (72 ± 26 min vs. 63 ± 25 min, p = 0.5) with similar FiO₂ at 4 h post-TLV (27 ± 6% vs. 33 ± 7%, p = 0.3) between the L-V_T and H-V_T lambs. No significant differences were measured in histological inflammation scores between L-V_T and H-V_T lambs, although lambs in both groups exhibited slightly higher scores than the control lambs. The L-V_T group displayed higher IL1B mRNA expression than the H-V_T group in both anterior (2.8 ± 1.5-fold increase vs. 1.3 ± 0.4-fold increase, p = 0.02) and posterior lung regions (3.0 ± 1.0-fold change increase vs. 1.1 ± 0.3-fold increase, p = 0.002), respectively. No significant differences were found in IL6 and TNF expression levels. Gene expression changes overall indicated that L-V_T was associated with a qualitatively distinct inflammatory gene expression profiles compared to H-V_T, which may indicate different clinical effects. In light of these findings, further mechanistic studies are warranted. In conclusion, we found no advantage of lower tidal volume use, which was in fact associated with a slightly unfavorable pattern of inflammatory gene expression.

Keywords: liquid ventilation; newborn lamb; tidal volume; transcriptome; ventilator-induced lung injury.