Detection and identification of Staphylococcus aureus using magnetic particle enhanced surface plasmon spectroscopy

Abstract

In this work, an approach for SPR spectroscopy using the ^liSPR system is examined that combines signal amplification by PCR and magnetic nanoparticles in one injection step. Therefore, the synthesis of PCR products was performed on the beads similar to a solid-phase PCR, termed PCR-on-a-bead. The functionality of this PCR was proven using an enzymatic assay. For validation the detection of oligonucleotides by SPR, an asymmetric PCR product was investigated. A signal increase upon binding of the PCR product to the specific probes was observed. In addition, surface regeneration of the chip was examined and reuse for at least two times ascertained. Amplification of the SPR signal by magnetic beads was verified but no signal was detected for PCR products immobilized on particles prior to injection.

Keywords: Magnetic nanoparticles; Signal amplification; Solid phase PCR; Staphylococcus aureus; Surface plasmon resonance.