Generation of human induced pluripotent stem cell line from Alzheimer's disease patient with PSEN2 N141I mutation using integration-free non-viral method

Harry Murti; Kelvin Pieknell; Indra Bachtiar; Yanuar Alan Sulistio; Sang-Hun Lee

doi:10.1016/j.scr.2020.101892

Generation of human induced pluripotent stem cell line from Alzheimer's disease patient with PSEN2 N141I mutation using integration-free non-viral method

Stem Cell Res. 2020 Jun 29:47:101892. doi: 10.1016/j.scr.2020.101892. Online ahead of print.

Authors

Harry Murti¹, Kelvin Pieknell², Indra Bachtiar¹, Yanuar Alan Sulistio³, Sang-Hun Lee⁴

Affiliations

¹ Department of Translational Research Stem Cell, Stem Cell and Cancer Institute, PT. Kalbe Farma Tbk., Jakarta, Indonesia; ReGeniC Laboratory, PT. Bifarma Adiluhung, Jakarta, Indonesia.
² Hanyang Biomedical Research Institute, Hanyang University, Seoul, Republic of Korea; Graduate School of Biomedical Science and Engineering, Hanyang University, Seoul, Republic of Korea.
³ Hanyang Biomedical Research Institute, Hanyang University, Seoul, Republic of Korea; Department of Biochemistry and Molecular Biology, College of Medicine, Hanyang University, Seoul, Republic of Korea. Electronic address: sulistio@hanyang.ac.kr.
⁴ Hanyang Biomedical Research Institute, Hanyang University, Seoul, Republic of Korea; Department of Biochemistry and Molecular Biology, College of Medicine, Hanyang University, Seoul, Republic of Korea; Graduate School of Biomedical Science and Engineering, Hanyang University, Seoul, Republic of Korea.

PMID: 32623353
DOI: 10.1016/j.scr.2020.101892

Abstract

Induced pluripotent stem cell (iPSC) line HUi001-A was reprogrammed from skin fibroblasts via non-integrating, virus free self-replicating RNA. Skin fibroblasts from an 81-year-old female Caucasian familial Alzheimer's disease patient of Volga German family carrying N141I mutation in the PSEN2 gene (familial AD4, clinical summary confirmed Alzheimer's disease) was obtained from the Coriell Institute (AG09908). Generated iPSCs were characterized and pluripotency was confirmed.