High resolution single particle Cryo-EM refinement using JSPR

Chen Sun; Brenda Gonzalez; Frank S Vago; Wen Jiang

doi:10.1016/j.pbiomolbio.2020.05.006

High resolution single particle Cryo-EM refinement using JSPR

Prog Biophys Mol Biol. 2021 Mar:160:37-42. doi: 10.1016/j.pbiomolbio.2020.05.006. Epub 2020 Jul 3.

Authors

Chen Sun¹, Brenda Gonzalez¹, Frank S Vago¹, Wen Jiang²

Affiliations

¹ Department of Biological Sciences, Markey Center for Structural Biology, Purdue University, West Lafayette, IN, 47906, USA.
² Department of Biological Sciences, Markey Center for Structural Biology, Purdue University, West Lafayette, IN, 47906, USA. Electronic address: jiang12@purdue.edu.

Abstract

JSPR is a single particle cryo-EM image processing and 3D reconstruction software developed in the Jiang laboratory at Purdue University. It began as a few refinement scripts for symmetric and asymmetric reconstructions of icosahedral viruses, but has grown into a comprehensive suite of tools for building ab initio reconstructions, high resolution refinements of viruses, protein complexes of arbitrary symmetries including helical tubes/filaments, and image file handling utilities. In this review, we will present examples achieved using JSPR and demonstrate recently implemented features of JSPR such as multi-aberration "alignments" and automatic optimization of masking for the assessment of map resolution using "true" FSC.

Keywords: Aberration correction; Beam tilt; Defocus refinement; Elliptic distortion; Generalized 2D alignments; Phase shift refinement.

Publication types

Research Support, N.I.H., Extramural
Review

MeSH terms

Bacteriophages / chemistry
Cryoelectron Microscopy / methods*
Enterovirus / chemistry
Image Processing, Computer-Assisted
Imaging, Three-Dimensional
Protein Conformation
Single Molecule Imaging / methods*
Software
Viruses / chemistry*
Zika Virus / chemistry

Abstract

Publication types

MeSH terms

Grants and funding