A simple and convenient electrochemiluminescence immunoassay by using gold nanoparticles as both label and co-reactant

Changzhi Zhao; Lulu Niu; Xiaoyu Wang; Wei Sun

doi:10.1016/j.bioelechem.2020.107585

A simple and convenient electrochemiluminescence immunoassay by using gold nanoparticles as both label and co-reactant

Bioelectrochemistry. 2020 Oct:135:107585. doi: 10.1016/j.bioelechem.2020.107585. Epub 2020 Jun 20.

Authors

Changzhi Zhao¹, Lulu Niu², Xiaoyu Wang², Wei Sun³

Affiliations

¹ Key Laboratory of Optic-electric Sensing and Analytical Chemistry for Life Science, The Ministry of Education, College of Chemistry & Molecular Engineering, Qingdao University of Science & Technology, Qingdao 266042, China; Key Laboratory of Laser Technology and Optoelectronic Functional Materials of Hainan Province, College of Chemistry and Chemical Engineering, Hainan Normal University, Haikou 571158, China. Electronic address: zhaocz@qust.edu.cn.
² Key Laboratory of Optic-electric Sensing and Analytical Chemistry for Life Science, The Ministry of Education, College of Chemistry & Molecular Engineering, Qingdao University of Science & Technology, Qingdao 266042, China.
³ Key Laboratory of Laser Technology and Optoelectronic Functional Materials of Hainan Province, College of Chemistry and Chemical Engineering, Hainan Normal University, Haikou 571158, China.

PMID: 32619749
DOI: 10.1016/j.bioelechem.2020.107585

Abstract

A simple and convenient electrochemiluminescence immunoassay (ECLIA) has been developed by using gold nanoparticles (AuNPs) as both label and co-reactant. In this novel ECLIA, the detection antibodies labeled with AuNPs as co-reactant reacts directly with ruthenium complex to produce electrochemiluminescence (ECL), which avoids the weakness of luminophore as a label. The feasibility of the new method was investigated through the sandwich ECL immunosensor for the determination of human immunoglobulin (HIgG), and excitation of luminescence was respectively driven by linear sweep voltammetry and step potential. Under the optimal conditions, ECL intensity of the immunosensor increased with HIgG concentration in a wide range from 0.01 to 10.0 ng/mL and displayed linear response to logarithm of HIgG concentration. The results showed that the detection limit was 5 pg/mL, and the relative error was no more than 4.6% for the repeated measurements. The relative standard deviation was less than 2.9% for the determination of the standard sample, which can meet the requirement of ECLIA.

Keywords: Co-reactants; Electrochemiluminescence; Gold nanoparticles; Immunoassay; Label; Ruthenium complex.

MeSH terms

Electrochemical Techniques / methods*
Gold / chemistry*
Immunoassay / methods*
Immunoglobulin G / analysis
Limit of Detection
Luminescence*
Metal Nanoparticles / chemistry*

Substances

Immunoglobulin G
Gold