Abstract
Mink enteritis virus (MEV) is a parvovirus that causes acute enteritis in mink. The capsid protein VP2 of MEV is a major immunogenicity that is important for disease prevention. In this study, this protein was expressed in Spodoptera frugiperda 9 cells using a recombinant baculovirus system and was observed to self-assemble into virus-like particles (VLPs) with a high hemagglutination (HA) titer (1:216). A single-dose injection of VLPs (HA titer, 1:256) resulted in complete protection of mink against virulent MEV challenge for at least 180 days. These data suggest that these MEV VLPs could be used as a vaccine for the prevention of viral enteritis in mink.
MeSH terms
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Animals
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Baculoviridae / genetics
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Baculoviridae / metabolism
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Capsid Proteins / administration & dosage
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Capsid Proteins / genetics*
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Capsid Proteins / immunology*
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Gene Expression
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Mink / immunology
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Mink / virology
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Mink Viral Enteritis / immunology
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Mink Viral Enteritis / prevention & control*
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Mink Viral Enteritis / virology
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Mink enteritis virus / genetics
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Mink enteritis virus / immunology*
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Mink enteritis virus / pathogenicity
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Recombinant Proteins / administration & dosage
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Recombinant Proteins / genetics
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Recombinant Proteins / immunology
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Sf9 Cells
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Spodoptera
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Viral Vaccines / administration & dosage
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Viral Vaccines / genetics
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Viral Vaccines / immunology
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Virulence
Substances
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Capsid Proteins
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Recombinant Proteins
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Viral Vaccines