miR-100-5p Inhibits Malignant Behavior of Chordoma Cells by Targeting IGF1R

Hongliang Zhang; Kang Yang; Tingting Ren; Yi Huang; Xin Liang; Yiyang Yu; Wei Wang; Jianfang Niu; Jingbing Lou; Xiaodong Tang; Wei Guo

doi:10.2147/CMAR.S252185

miR-100-5p Inhibits Malignant Behavior of Chordoma Cells by Targeting IGF1R

Cancer Manag Res. 2020 Jun 2:12:4129-4137. doi: 10.2147/CMAR.S252185. eCollection 2020.

Authors

Hongliang Zhang^{1

2}, Kang Yang³, Tingting Ren^{1

2}, Yi Huang^{1

2}, Xin Liang^{1

2}, Yiyang Yu^{1

2}, Wei Wang^{1

2}, Jianfang Niu^{1

2}, Jingbing Lou^{1

2}, Xiaodong Tang^{1

2}, Wei Guo^{1

2}

Affiliations

¹ Musculoskeletal Tumor Center, Peking University People's Hospital, Beijing, People's Republic of China.
² Beijing Key Laboratory of Musculoskeletal Tumor, Beijing, People's Republic of China.
³ Department of Orthopedics, Yangzhou University Affiliated Hospital, Yangzhou, People's Republic of China.

Abstract

Purpose: Our research aimed to illuminate the role of miR-100-5p in chordoma and potential mechanism.

Materials and methods: We used microRNA array analysis to explore differentially expressed miRNAs in chordoma tissue and then verified by qRT-PCR. Cell proliferation and transwell assay were used to evaluate the function of miR-100-5p. Cell apoptosis was analyzed by flow cytometry, and using biological software, we predicted that the insulin-like growth factor 1 receptor (IGF1R) could be the target gene of miR-100-5p, which was then validated by dual luciferase assays and Western blot.

Results: miR-100-5p was downregulated in chordoma tissues. Overexpression of miR-100-5p could suppress the growth of chordoma both in vitro and in vivo, and miR-100-5p could inhibit the migration and invasion of chordoma cells partially by suppressing epithelial-mesenchymal transition (EMT). Furthermore, IGF1R was validated as the target gene of miR-100-5p and expressed in most chordoma tissues.

Conclusion: In conclusion, our results showed that miR-100-5p was lowly expressed in chordoma and inhibited tumor malignant progression by targeting IGF1R.

Keywords: IGF1R; chordoma; miR-100-5p; miRNA.