The aim of this research is to introduce a novel label free electrochemical immunosensor based on glassy carbon electrode (GCE) modified with carboxylated carbon nanotubes (COOH-MWCNTs)/polyaniline (PANI)/gold nanoparticles (AuNPs) for the detection of prostate specific antigen (PSA). The AuNPs were utilized as a connector for PSA antibody immobilization through NH2 groups on antibody. Investigations on modified electrode surface were performed by FT-IR spectrum, scanning electron microscope (SEM) and energy dispersive X-ray spectroscopy (EDS) to evaluate the synthesized nanocomposite and modified electrode surface. As a sensitive analytical method for the detection of PSA, differential pulse voltammetry (DPV) was employed in different ranges of antigen concentration, 1.66 ag·mL-1 to 1.3 ng·mL-1. In addition, the detection limit was obtained 0.5 pg·mL-1, from the linear relationship between antigen concentration log and peak current. Also, the proposed immunosensor was carried out for the determination of PSA in human serum samples, indicating recoveries ranging from 92 to 104%. Finally, it should be noted that the reproducibility and specificity, along with the stability of the present immunosensor were examined, and satisfactory findings were obtained, thus proving it as a promising PSA immunosensor.
Keywords: Functionalized multi-walled carbon nanotubes; Gold nanoparticles; Immunosensor; Polyaniline; Prostate specific antigen.
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