Complete Photochemical Regulation of 8-17 DNAzyme Activity by Using Reversible DNA Photo-crosslinking

Yasuha Watanabe; Kenzo Fujimoto

doi:10.1002/cbic.202000227

Complete Photochemical Regulation of 8-17 DNAzyme Activity by Using Reversible DNA Photo-crosslinking

Chembiochem. 2020 Nov 16;21(22):3244-3248. doi: 10.1002/cbic.202000227. Epub 2020 Jul 23.

Authors

Yasuha Watanabe¹, Kenzo Fujimoto¹

Affiliation

¹ School of Advanced Science and Technology, Japan Advanced Institute Science and Technology, Asahidai 1-1, Nomi, Ishikawa, 923-1292, Japan.

PMID: 32596920
DOI: 10.1002/cbic.202000227

Abstract

The regulation of DNAzyme activity is an important problem for its in vivo applications. We achieved photochemical regulation of DNAzyme activity by using reversible DNA photo-crosslinking of 3-cyanovinylcarbazole (^CNV K). The ODN containing ^CNV K photo-crosslinked to a pyrimidine base in the complementary strand after a few seconds of photoirradiation, and its photoadduct was split by photoirradiation of another wavelength. The activity of photo-crosslinked DNAzyme with ^CNV K was completely inhibited (OFF state). In contrast, after 312 nm irradiation, DNAzyme activity was recovered upon addition of a substrate strand (ON state). In addition, the photo-crosslinked DNAzyme is prone to enzymatic digestion by exonuclease. This photochemical OFF to ON switching with reversible DNA photo-crosslinking was regulated at the desired time and position; therefore, it might be possible to use it for in vivo application.

Keywords: DNAzymes; cyanovinylcarbazole; enzyme resistance; photo-chemical switching; reversible photo-crosslinking.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Cross-Linking Reagents / chemistry
Cross-Linking Reagents / metabolism*
DNA / chemistry
DNA / metabolism*
DNA, Catalytic / chemistry
DNA, Catalytic / metabolism*
Photochemical Processes

Substances

Cross-Linking Reagents
DNA, Catalytic
DNA