Simultaneous Nanothermometry and Deep-Tissue Imaging

Abstract

Bright, stable, and biocompatible fluorescent contrast agents operating in the second biological window (1000-1350 nm) are attractive for imaging of deep-lying structures (e.g., tumors) within tissues. Ideally, these contrast agents also provide functional insights, such as information on local temperature. Here, water-dispersible barium phosphate nanoparticles doped with Mn⁵⁺ are made by scalable, continuous, and sterile flame aerosol technology and explored as fluorescent contrast agents with temperature-sensitive peak emission in the NIR-II (1190 nm). Detailed assessment of their stability, toxicity with three representative cell lines (HeLa, THP-1, NHDF), and deep-tissue imaging down to about 3 cm are presented. In addition, their high quantum yield (up to 34%) combined with excellent temperature sensitivity paves the way for concurrent deep-tissue imaging and nanothermometry, with biologically well-tolerated nanoparticles.

Keywords: Mn5+‐doped Ba3(PO4)2; bioimaging; cytocompatibility; flame spray pyrolysis; intravital microscopy.