Herein, a surface plasmon resonance (SPR) enhanced DNA biosensor has been developed for real-time detection of donkey meat marker using biotinylated reporter and streptavidin functionalized gold nanostars (Stre@GNSs). Compared to the direct detection assay, this sandwich format for the enhancement of the signal, resulted in 6-folds orders increase in the sensitivity. Target DNA could be detected with the lower limit of quantification (LLOQ) of 1.0 nM with a relative standard deviation (RSD, n = 3) of 0.85%. In addition, the fabricated SPR sensor showed good selectivity for the target analyte over full complementary, single-base mismatch, three base-mismatch and non-complementary oligonucleotides. Finally, the proposed sensor was successfully applied for detection of donkey meat adulteration with various percentages in homemade beef sausage, as a real sample. The results indicated that the proposed biosensor provides a high specificity, easy, good sensitivity and fast approach for identification of donkey meat adulteration in food samples.
Keywords: DNA detection; Donkey meat adulteration; SPR biosensor; Signal enhancement; Streptavidinylated gold nanostars.
Copyright © 2020. Published by Elsevier Ltd.