Overexpression of SFA1 in engineered Saccharomyces cerevisiae to increase xylose utilization and ethanol production from different lignocellulose hydrolysates

Lang Zhu; Pengsong Li; Tongming Sun; Meilin Kong; Xiaowei Li; Sajid Ali; Wenbo Liu; Sichun Fan; Jingchun Qiao; Shizhong Li; Liangcai Peng; Boyang He; Mingjie Jin; Wei Xiao; Limin Cao

doi:10.1016/j.biortech.2020.123724

Overexpression of SFA1 in engineered Saccharomyces cerevisiae to increase xylose utilization and ethanol production from different lignocellulose hydrolysates

Bioresour Technol. 2020 Oct:313:123724. doi: 10.1016/j.biortech.2020.123724. Epub 2020 Jun 21.

Authors

Lang Zhu¹, Pengsong Li², Tongming Sun¹, Meilin Kong¹, Xiaowei Li¹, Sajid Ali¹, Wenbo Liu¹, Sichun Fan¹, Jingchun Qiao¹, Shizhong Li³, Liangcai Peng⁴, Boyang He⁴, Mingjie Jin⁵, Wei Xiao⁶, Limin Cao⁷

Affiliations

¹ College of Life Sciences, Capital Normal University, Beijing 100048, China.
² Beijing Key Laboratory for Source Control Technology of Water Pollution, Engineering Research Center for Water Pollution Source Control and Eco-remediation, College of Environmental Science and Engineering, Beijing Forestry University, Beijing 100083, China.
³ MOST-USDA Joint Research Center for Biofuels, Beijing Engineering Research Center for Biofuels, Institute of New Energy Technology, Tsinghua University, Beijing 100084 China.
⁴ Biomass and Bioenergy Research Centre, Huazhong Agricultural University, Wuhan 430070, China; College of Plant Science and Technology, Huazhong Agricultural University, Wuhan 430070, China.
⁵ School of Environmental and Biological Engineering, Nanjing University of Science and Technology, Nanjing 210094, China.
⁶ College of Life Sciences, Capital Normal University, Beijing 100048, China; Department of Biochemistry, Microbiology and Immunology, University of Saskatchewan, Saskatoon, SK S7N 5E5, Canada.
⁷ College of Life Sciences, Capital Normal University, Beijing 100048, China. Electronic address: caolimin@cnu.edu.cn.

PMID: 32586644
DOI: 10.1016/j.biortech.2020.123724

Abstract

Here, an engineered Saccharomyces cerevisiae strain SFA1^OE was constructed by overexpressing SFA1 in a reported WXY70 with effective six-gene clusters. Under simulated maize hydrolysate, SFA1^OE produced an ethanol yield of 0.492 g/g totalsugars within 48 h. The productivity of SFA1^OE was comprehensively evaluated in typical hydrolysates from stalks of maize, sweet sorghum, wheat and Miscanthus. Within 48 h, SFA1^OE achieved an ethanol yield of 0.489 g/g totalsugars in the optimized hydrolysate of alkaline-distilled sweet sorghum bagasse derived from Advanced Solid-State Fermentation process. By crossing SFA1^OE with a DQ1-derived haploid strain, we obtained an evolved diploid strain SQ-2, exhibiting improved ethanol production and thermotolerance. This study demonstrates that overexpressing SFA1 enables efficient fermentation performance in different lignocellulosic hydrolysates, especially in the hydrolysate of alkaline-distilled sweet sorghum bagasse. The increased cellulosic bioethanol production of SFA1^OE provides a promising platform for efficient biorefineries.

Keywords: Ethanol; Lignocellulosic; SFA1; Saccharomyces cerevisiae; Xylose.

MeSH terms

Ethanol
Fermentation
Hydrolysis
Lignin
Saccharomyces cerevisiae*
Xylose*

Substances

lignocellulose
Ethanol
Lignin
Xylose