Physicochemical properties of dentinogenesis imperfecta with a known DSPP mutation

Haemin Park; Hong-Keun Hyun; Kyung Mi Woo; Jung-Wook Kim

doi:10.1016/j.archoralbio.2020.104815

Physicochemical properties of dentinogenesis imperfecta with a known DSPP mutation

Arch Oral Biol. 2020 Sep:117:104815. doi: 10.1016/j.archoralbio.2020.104815. Epub 2020 Jun 13.

Authors

Haemin Park¹, Hong-Keun Hyun¹, Kyung Mi Woo², Jung-Wook Kim³

Affiliations

¹ Department of Pediatric Dentistry, School of Dentistry and Dental Research Institute, Seoul National University, Seoul, Republic of Korea.
² Department of Molecular Genetics, School of Dentistry and Dental Research Institute, BK21 Program, Seoul National University, Seoul, Republic of Korea.
³ Department of Pediatric Dentistry, School of Dentistry and Dental Research Institute, Seoul National University, Seoul, Republic of Korea; Department of Molecular Genetics, School of Dentistry and Dental Research Institute, BK21 Program, Seoul National University, Seoul, Republic of Korea. Electronic address: pedoman@snu.ac.kr.

PMID: 32585446
DOI: 10.1016/j.archoralbio.2020.104815

Abstract

Aim: To investigate the chemical and mechanical properties of teeth affected by a 1-bp deletion (c.2688delT) in the DSPP gene.

Methods and materials: Maxillary first premolars were extracted from the affected individual at age 9 years due to the orthodontic reason for crowding. A sample was imbedded in epoxy resin and sectioned buccolingually, after micro-computerized tomography (μCT) images were taken. Scanning Electron Microscopy (SEM), Energy Dispersive Spectrometry (EDS) and Vickers microhardness testing were also performed.

Results: μCT reconstruction and analysis showed an irregularly obliterated pulp chamber and an extremely small pulpal volume in the DGI-II sample. The mineral density and microhardness scores were smaller in the dentin of the DGI-II sample compared to the wild-type. Mg content was lower in the dentin of the DGI-II sample compared to the wild-type.

Conclusion: This study shows that dentin affected by a 1-bp deletion in DSPP has a reduced mineral density, diminished microhardness and reduced Mg content.

Keywords: DSPP; Dentinogenesis imperfecta; EDS; MicroCT; Microhardness; Mutation.

MeSH terms

Child
Dentin / pathology
Dentinogenesis Imperfecta* / diagnostic imaging
Dentinogenesis Imperfecta* / genetics
Extracellular Matrix Proteins / genetics*
Humans
Mutation
Pedigree
Phosphoproteins / genetics*
Sequence Deletion
Sialoglycoproteins / genetics*

Substances

Extracellular Matrix Proteins
Phosphoproteins
Sialoglycoproteins
dentin sialophosphoprotein