Abstract
A facile enzymatic modular assembly strategy for the preparative-scale synthesis of poly-N-acetyllactosamine (poly-LacNAc) glycans with varied lengths and designed sialylation and/or fucosylation patterns is described. These glycans were printed as a microarray to investigate their interactions with a panel of glycan binding proteins (GBPs). Binding affinities revealed that the avidity of GBPs could be largely affected by the length and the patterns of sialylation and fucosylation.
MeSH terms
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Ascomycota / chemistry
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Bacteria / enzymology
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Bacterial Proteins / chemistry
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Carbohydrate Sequence
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E-Selectin / metabolism
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Glycosylation
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Glycosyltransferases / chemistry*
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Griffonia / chemistry
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Hemagglutinins / metabolism
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Humans
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Lectins / metabolism
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Maackia / chemistry
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Microarray Analysis
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Molecular Structure
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Plant Lectins / metabolism
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Polysaccharides / chemical synthesis*
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Polysaccharides / metabolism
Substances
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Bacterial Proteins
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E-Selectin
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Griffonia simplicifolia lectins
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Hemagglutinins
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Lectins
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Plant Lectins
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Polysaccharides
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SELE protein, human
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lectin, Aleuria aurantia
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poly-N-acetyllactosamine
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Glycosyltransferases