Suppressive effects of 2-methacryloyloxyethyl phosphorylcholine (MPC)-polymer on the adherence of Candida species and MRSA to acrylic denture resin

Natsumi Fujiwara; Keiji Murakami; Kaya Yoshida; Shunsuke Sakurai; Yasusei Kudo; Kazumi Ozaki; Katsuhiko Hirota; Hideki Fujii; Maiko Suzuki; Yoichiro Miyake; Hiromichi Yumoto

doi:10.1016/j.heliyon.2020.e04211

Suppressive effects of 2-methacryloyloxyethyl phosphorylcholine (MPC)-polymer on the adherence of Candida species and MRSA to acrylic denture resin

Heliyon. 2020 Jun 16;6(6):e04211. doi: 10.1016/j.heliyon.2020.e04211. eCollection 2020 Jun.

Authors

Affiliations

¹ Department of Oral Health Care Promotion, Institute of Biomedical Sciences, Tokushima University Graduate School, 3-18-15 Kuramoto, Tokushima 770-8504, Japan.
² Department of Oral Biology and Diagnostic Sciences, The Dental College of Georgia, Augusta University, 1120 15th Street, Augusta, GA, 30912, United States.
³ Department of Oral Microbiology, Institute of Biomedical Sciences, Tokushima University Graduate School, 3-18-15 Kuramoto, Tokushima 770-8504, Japan.
⁴ Department of Oral Health Care Education, Institute of Biomedical Sciences, Tokushima University Graduate School, 3-18-15 Kuramoto, Tokushima 770-8504, Japan.
⁵ Life Science Products Division, NOF Corporation, 4-20-3 Ebisu, Shibuya, Tokyo 150-6019, Japan.
⁶ Department of Oral Molecular Pathology, Institute of Biomedical Sciences, Tokushima University Graduate School, 3-18-15 Kuramoto, Tokushima 770-8504, Japan.
⁷ Department of Medical Hygiene, Dental Hygiene Course, Kochi Gakuen College, 292-26 Asahi tenjin, Kochi 780-0955, Japan.
⁸ Department of Oral Health Sciences, Faculty of Health and Welfare, Tokushima Bunri University, 180 Nishihama-Boji, Yamashiro, Tokushima 770-8514, Japan.
⁹ Department of Periodontology and Endodontology, Institute of Biomedical Sciences, Tokushima University Graduate School, 3-18-15 Kuramoto, Tokushima 770-8504, Japan.

Abstract

Objectives: The effects of 2-methacryloyloxyethyl phosphorylcholine (MPC)-polymer on the adherence of microorganisms such as non-Candida albicans Candida (NCAC) and methicillin-resistant Staphylococcus aureus (MRSA), frequently detected in oral infections in immunocompromised and/or elderly people, to denture resin material, are still unclear. Here, we report the effects of MPC-polymer on the adherence of C. albicans, NCAC, and MRSA to acrylic denture resin.

Methods: Sixteen strains of C. albicans, seven strains of C. glabrata, two strains of C. tropicalis, one strain of C. parapsilosis, and six strains of MRSA were used. We cultured the fungal/bacterial strains and examined the cell growth and adherence of fungi/bacteria to mucin-coated acrylic denture resin plates (ADRP) with or without MPC-polymer coating, by scanning electron microscopy. The cell surface hydrophobicity of the fungal/bacterial strains was measured by the adsorption to hydrocarbons.

Results: MPC-polymer did not affect the growth of all strains of Candida species and MRSA, but significantly suppressed adherence to ADRP in most strains of C. albicans and all strains of NCAC and MRSA. A significant positive correlation was found between cell hydrophobicity and the reduction rates of microbial adherence to ADRP treated with 5% of MPC-polymer.

Conclusions: MPC-polymer treatment for acrylic resin material suppresses the adherence of C. albicans, NCAC and MRSA via their hydrophilicity interaction.

Clinical significance: The application of MPC-polymer for denture hygiene is potent to prevent oral candidiasis, denture stomatitis and opportunistic infection, caused by Candida species and MRSA, via suppressing the adherence of those fungus/bacteria.

Keywords: 2-Methacryloyloxyethyl phosphorylcholine; Candida; Dental materials; Dentistry; Denture; Hydrophilicity; MRSA; Medical microbiology; Microbiology; Oral medicine; Prosthetic dentistry.