Antioxidant activities of Clerodendrum cyrtophyllum Turcz leaf extracts and their major components

PLoS One. 2020 Jun 23;15(6):e0234435. doi: 10.1371/journal.pone.0234435. eCollection 2020.

Abstract

This study was designed to investigate the antioxidant properties of the extracts and subfractions of various polarities from Clerodendrum cyrtophyllum Turcz leaves and the related phenolic compound profiles. The ethyl acetate fraction (EAF) showed the most potent radical-scavenging activity for DPPH radicals, ABTS radicals, and superoxide anion (O2·-) radicals as well as the highest reducing power of the fractions tested; the n-butyl alcohol fraction (BAF) was the most effective in scavenging hydroxyl radical (OH·), and the dichloromethane fraction (DMF) exhibited the highest ferrous ion chelating activity. Twelve phenolic components were identified from the EAF of C. cyrtophyllum. Additionally, acteoside (1) was found to be a major component (0.803 g, 0.54%) and show DPPH and ABTS radical scavenging activities with IC50 values of 79.65±3.4 and 23.00±1.5 μg/ml, indicating it is principally responsible for the significant total antioxidant effect of C. cyrtophyllum. Our work offers a theoretical basis for further utilization of C. cyrtophyllum as a potential source of natural, green antioxidants derived from plants.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Antioxidants / analysis
  • Antioxidants / isolation & purification
  • Antioxidants / pharmacology*
  • Clerodendrum / chemistry*
  • Glucosides / analysis
  • Glucosides / isolation & purification
  • Glucosides / pharmacology*
  • Hydroxyl Radical / metabolism
  • Phenols / analysis
  • Phenols / isolation & purification
  • Phenols / pharmacology*
  • Plant Extracts / analysis
  • Plant Extracts / isolation & purification
  • Plant Extracts / pharmacology*
  • Plant Leaves / chemistry
  • Superoxides / metabolism

Substances

  • Antioxidants
  • Glucosides
  • Phenols
  • Plant Extracts
  • Superoxides
  • Hydroxyl Radical
  • acteoside

Grants and funding

This work was financially supported by the National Natural Science Foundation of China (No. 81973229/81660584), Key Research and Development Program of Hainan Province (ZDYF2017099), Key Project of Education Department of Hainan Province (Hnky2019ZD-6) and Research initiation fund project of Hainan University (No.KYQD(ZR)20001).This work was financially supported by the High-level Talents Programs of Hainan Province (No. 2019RC006).