CRISPR adaptation from a structural perspective

Giedrius Sasnauskas; Virginijus Siksnys

doi:10.1016/j.sbi.2020.05.015

CRISPR adaptation from a structural perspective

Curr Opin Struct Biol. 2020 Dec:65:17-25. doi: 10.1016/j.sbi.2020.05.015. Epub 2020 Jun 20.

Authors

Giedrius Sasnauskas¹, Virginijus Siksnys²

Affiliations

¹ Institute of Biotechnology, Vilnius University, Sauletekio Av. 7, Vilnius 10257, Lithuania.
² Institute of Biotechnology, Vilnius University, Sauletekio Av. 7, Vilnius 10257, Lithuania. Electronic address: siksnys@ibt.lt.

PMID: 32570107
DOI: 10.1016/j.sbi.2020.05.015

Abstract

Bacterial CRISPR-Cas systems provide adaptive immunity against viruses and other mobile genome elements. During the adaptation step cells become immunized by insertion of short fragments of foreign DNA, termed spacers, into the genomic region called a CRISPR array. Selection, processing and insertion of new spacers is an elaborate and precisely orchestrated reaction, which relies on the Cas1-Cas2 integrase complex and accessory proteins that vary among different types of CRISPR-Cas systems. This review focuses on CRISPR adaptation from the structural perspective, with the spotlight on adaptation proteins employed by type I and type II CRISPR-Cas systems.

Publication types

Research Support, Non-U.S. Gov't
Review

MeSH terms

Bacterial Proteins* / metabolism
CRISPR-Associated Proteins / metabolism*
CRISPR-Cas Systems*
DNA Viruses / metabolism*
Escherichia coli / genetics*
Escherichia coli / virology
Host Microbial Interactions
Protein Binding
Viruses* / genetics

Substances

Bacterial Proteins
CRISPR-Associated Proteins