Structure of the O-specific polysaccharide from Azospirillum formosense CC-Nfb-7(T)

Elena N Sigida; Alexander S Shashkov; Evelina L Zdorovenko; Svetlana A Konnova; Yulia P Fedonenko

doi:10.1016/j.carres.2020.108060

Structure of the O-specific polysaccharide from Azospirillum formosense CC-Nfb-7(T)

Carbohydr Res. 2020 Aug:494:108060. doi: 10.1016/j.carres.2020.108060. Epub 2020 Jun 10.

Authors

Elena N Sigida¹, Alexander S Shashkov², Evelina L Zdorovenko², Svetlana A Konnova³, Yulia P Fedonenko³

Affiliations

¹ Institute of Biochemistry and Physiology of Plants and Microorganisms, Russian Academy of Sciences, 13 Prospekt Entuziastov, Saratov, 410049, Russia. Electronic address: si_elena@mail.ru.
² N. D. Zelinsky Institute of Organic Chemistry, Russian Academy of Sciences, 47 Leninsky Prospekt, Moscow, 119991, Russia.
³ Institute of Biochemistry and Physiology of Plants and Microorganisms, Russian Academy of Sciences, 13 Prospekt Entuziastov, Saratov, 410049, Russia; Saratov State University, 83 Ulitsa Astrakhanskaya, Saratov, 410012, Russia.

PMID: 32569849
DOI: 10.1016/j.carres.2020.108060

Abstract

The lipopolysaccharide was obtained from the cells of Azospirillum formosense CC-Nfb-7(T), a diazotrophic bacterium isolated from agricultural soil. The O-specific polysaccharide (OPS) was released by mild acid hydrolysis of the lipopolysaccharide and was studied by sugar analysis along with ¹H and ¹³C NMR spectroscopy, including ¹H,¹H COSY, TOCSY, ROESY, ¹H,¹³C HSQC, and HMBC experiments, and Smith degradation. The following structure of partially methylated OPS composed of trisaccharide repeating units was established.

Keywords: 3-O-Methyl-d-mannose; Azospirillum formosense; Lipopolysaccharide; O-specific polysaccharide; Structure.

MeSH terms

Azospirillum / chemistry*
Azospirillum / cytology
Azospirillum / metabolism
Hydrolysis
Polysaccharides / chemistry*
Polysaccharides / metabolism

Substances

Polysaccharides

Supplementary concepts

Azospirillum formosense