mRNA-Based Reprogramming Under Xeno-Free and Feeder-Free Conditions

Jakob Jeriha; Nikola Kolundzic; Preeti Khurana; Andrea Perez-Dominguez; Dusko Ilic

doi:10.1007/7651_2020_302

mRNA-Based Reprogramming Under Xeno-Free and Feeder-Free Conditions

Methods Mol Biol. 2022:2454:665-674. doi: 10.1007/7651_2020_302.

Authors

Jakob Jeriha¹, Nikola Kolundzic², Preeti Khurana², Andrea Perez-Dominguez², Dusko Ilic²

Affiliations

¹ Stem Cell Laboratory, Assisted Conception Unit, School of Life Course Sciences, Department of Women and Children's Health, Faculty of Life Sciences & Medicine, King's College London, London, UK. jakob.jeriha@kcl.ac.uk.
² Stem Cell Laboratory, Assisted Conception Unit, School of Life Course Sciences, Department of Women and Children's Health, Faculty of Life Sciences & Medicine, King's College London, London, UK.

PMID: 32567019
DOI: 10.1007/7651_2020_302

Abstract

Reprogramming somatic cells into induced pluripotent stem cells (iPSC) has provided a gateway for many novel discoveries in the field of tissue engineering, regenerative medicine and cell therapy. The need for an efficient, less laborious and fast reprogramming protocol under xeno-free, feeder-free and chemically defined conditions has never been greater. Here we describe a novel approach to reprogramming using the StemRNA 3rd Gen Reprogramming Kit (ReproCELL) which encompasses non-modified microRNAs (NM-miRNA), non-modified E3, K3, B18R RNAs (EKB NM-RNA) and non-modified mRNAs for six crucial transcription factors (OSKMNL NM-RNA).

Keywords: Chemically defined; Feeder-free; Reprogramming; Xeno-free; iPSC; mRNA; miRNA.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Cell Differentiation / genetics
Cellular Reprogramming* / genetics
Fibroblasts
Induced Pluripotent Stem Cells*
RNA, Messenger / genetics
Regenerative Medicine

Substances

RNA, Messenger