Inhibitory Activity of Boesenbergia rotunda (L.) Mansf. Rhizome towards the Expression of Akt and NF-KappaB p65 in Acetic Acid-Induced Wistar Rats

Aziiz Mardanarian Rosdianto; Irma Melyani Puspitasari; Ronny Lesmana; Jutti Levita

doi:10.1155/2020/6940313

Inhibitory Activity of Boesenbergia rotunda (L.) Mansf. Rhizome towards the Expression of Akt and NF-KappaB p65 in Acetic Acid-Induced Wistar Rats

Evid Based Complement Alternat Med. 2020 May 20:2020:6940313. doi: 10.1155/2020/6940313. eCollection 2020.

Authors

Aziiz Mardanarian Rosdianto^{1

2}, Irma Melyani Puspitasari^{1

3}, Ronny Lesmana^{4

5}, Jutti Levita¹

Affiliations

¹ Department of Pharmacology and Clinical Pharmacy, Faculty of Pharmacy, Universitas Padjadjaran, Sumedang 45363, Indonesia.
² Veterinary Medicine Program, Faculty of Medicine, Universitas Padjadjaran, Sumedang 45363, Indonesia.
³ Center of Excellence in Higher Education for Pharmaceutical Care Innovation, Universitas Padjadjaran, Sumedang 45363, Indonesia.
⁴ Physiology Molecular Laboratory, Central Laboratory, Universitas Padjadjaran, Sumedang 45363, Indonesia.
⁵ Department of Medical Basic Sciences, Faculty of Medicine, Universitas Padjadjaran, Sumedang 45363, Indonesia.

Abstract

Materials and methods: Forty-eight male Wistar rats were divided into anti-inflammatory mechanism study (n = 18) and acute toxicity study (n = 30). The anti-inflammatory mechanism study employed six groups (n = 3), e.g., the normal control, negative control, positive control (quercetin 20 mg/kg BW), and three doses of BREE (250 mg/kg BW; 500 mg/kg BW; 1000 mg/kg BW). All groups (except the normal control) were inflammatory-induced i.p. using 0.1 mL of 1% of acetic acid. The expression of Akt and NF-kappaB p65 in the stomach and intestine of the rats was examined using Western blot analysis. The acute toxicity study (21 days) was conducted by following the Regulation of Indonesia National Agency of Drug and Food Control No. 7/2014 about In Vivo Nonclinical Toxicity Study using 5 doses of BREE (250 mg/kg BW; 500 mg/kg BW; 1000 mg/kg BW; 2000 mg/kg BW; 4000 mg/kg BW).

Results: BREE reduces the infiltration of inflammatory cells in both the stomach and the intestine of acetic acid-induced rats. BREE also alters the expression of Akt and NF-kappaB p65 in the rat's stomach and intestine (p=0.005). The acute toxicity study reveals no lethal effects and behavioral signs of toxicity at all tested doses, which indicates that the LD₅₀ is greater than 4000 mg/kg BW.

Conclusion: Taken together, BREE could inhibit the expression of Akt and NF-kappaB p65 in the stomach and intestine of acetic acid-induced Wistar rats. This plant could be further explored for its potential as plant-based antistomach ulceration.