Exosomes carry IL-10 and antigen/MHC II complexes to induce antigen-specific oral tolerance

Hao-Tao Zeng; Jiang-Qi Liu; Miao Zhao; Dian Yu; Gui Yang; Li-Hua Mo; Zhi-Qiang Liu; Shuai Wang; Zhi-Gang Liu; Ping-Chang Yang

doi:10.1016/j.cyto.2020.155176

Exosomes carry IL-10 and antigen/MHC II complexes to induce antigen-specific oral tolerance

Cytokine. 2020 Sep:133:155176. doi: 10.1016/j.cyto.2020.155176. Epub 2020 Jun 18.

Authors

Hao-Tao Zeng¹, Jiang-Qi Liu¹, Miao Zhao¹, Dian Yu², Gui Yang³, Li-Hua Mo², Zhi-Qiang Liu¹, Shuai Wang⁴, Zhi-Gang Liu⁵, Ping-Chang Yang⁶

Affiliations

¹ Department of Allergy, Longgang ENT Hospital & Shenzhen Key Laboratory of ENT, Institute of ENT, Shenzhen, China.
² Research Center of Allergy & Immunology, Shenzhen University School of Medicine, Shenzhen, China.
³ Department of Otolaryngology, Longgang Central Hospital, Shenzhen, China.
⁴ Department of Allergy, Longgang ENT Hospital & Shenzhen Key Laboratory of ENT, Institute of ENT, Shenzhen, China. Electronic address: ws_only@hotmail.com.
⁵ Research Center of Allergy & Immunology, Shenzhen University School of Medicine, Shenzhen, China. Electronic address: lzg@suz.edu.cn.
⁶ Research Center of Allergy & Immunology, Shenzhen University School of Medicine, Shenzhen, China; Guangdong Provincial Key Laboratory of Regional Immunity and Diseases, Shenzhen, China. Electronic address: pcy2356@szu.edu.cn.

PMID: 32563958
DOI: 10.1016/j.cyto.2020.155176

Abstract

Background: It is known that the immune tolerance can be naturally established in the intestine, while the mechanism by which the immune tolerance development in the intestine is not fully understood yet. Vasoactive intestinal peptides (VIP) has the immune regulatory functions. This study aims to investigate the role of VIP in the immune tolerance development in the intestine.

Methods: Intestinal epithelial cell (IEC)-derived exosomes were prepared. The exosomes carried IL-10 and antigen/MHC II complexes. VIP-deficient (VIPd) mice and wild type mice were employed to test the role of VIP in the development of immune tolerance in the intestine.

Results: VIPd mice failed to induce type 1 regulatory T cells (Tr1 cells) in the intestine and retarded the establishment of antigen (Ag)-specific immune tolerance. Exposure to VIP in the culture induced IL-10 expression in intestinal epithelial cells (IECs). Exosomes derived from ovalbumin (OVA, used as a specific Ag)/VIP-primed IECs carried IL-10 and OVA/MHC II complexes; these exosomes were designated IL10CARs (IL-10/chimeric antigen receptor-carrying exosomes). IL10CARs could recognize OVA-specific CD4⁺ T cells and converted OVA-specific CD4^± T cells to OVA-specific Tr1 cells. Administration of IL10CARs suppressed experimental food allergy.

Conclusions: The data show that IL10CARs are capable of suppressing experimental FA by inducing antigen-specific Tr1 cells, which has the translation potential for FA treatment.

Keywords: Epithelial cells; Food allergy; Inflammation; Intestine; Vasoactive intestinal peptide.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Animals
Antigens / immunology*
CD4-Positive T-Lymphocytes / immunology
Epithelial Cells / immunology
Exosomes / immunology*
Food Hypersensitivity / immunology
Histocompatibility Antigens Class II / immunology*
Immune Tolerance / immunology*
Interleukin-10 / immunology*
Intestinal Mucosa / immunology*
Mice
Mice, Inbred BALB C
Ovalbumin / immunology
T-Lymphocytes, Regulatory / immunology
Vasoactive Intestinal Peptide / immunology

Substances

Antigens
Histocompatibility Antigens Class II
IL10 protein, mouse
Interleukin-10
Vasoactive Intestinal Peptide
Ovalbumin