In the present study, sheep manure (0%, 10% and 20% w/w) and biochar derived from coniferous tree woods (0%, 2.5% and 5% w/w) were incorporated into a multi-MTE contaminated soil from a former iron mine site and incubated for 10 days. A seeds of barley were grown in the amended soil and different morphological traits were measured after 30 days. Results indicated that MTE stress reduced the shoot length, stem diameter, leaf area, number of leaves and dry biomass as compared to the control. Organic amendments application increased soil pH and was found to affect significantly almost all the measured parameters. Animal manure was found effective in improvement of the morphological characteristics of barley plants comparing to biochar amendments. Our results suggested that animal manure could be used for reducing the effect of MTE on the morphological proprieties of barley grown in a former iron mine soil.
Keywords: Contaminated soil; Metal toxicity; Mine area; Morphological traits; Plant improvement.