Salivary Amylase Gene Copy Number Is Associated with the Obesity and Inflammatory Markers in Children

Vaithinathan Selvaraju; Chandra M K Venkatapoorna; Jeganathan R Babu; Thangiah Geetha

doi:10.2147/DMSO.S251359

Salivary Amylase Gene Copy Number Is Associated with the Obesity and Inflammatory Markers in Children

Diabetes Metab Syndr Obes. 2020 May 19:13:1695-1701. doi: 10.2147/DMSO.S251359. eCollection 2020.

Authors

Vaithinathan Selvaraju¹, Chandra M K Venkatapoorna¹, Jeganathan R Babu^{1

2}, Thangiah Geetha^{1

2}

Affiliations

¹ Department of Nutrition, Dietetics, and Hospitality Management, Auburn University, Auburn, AL, USA.
² Boshell Metabolic Diseases and Diabetes Program, Auburn University, Auburn, AL, USA.

Abstract

Background: We have recently shown that the copy number of salivary amylase (AMY1) gene was significantly decreased, and the obesity-related salivary biomarkers resistin, MCP-1, TNF-α, IL-6, and CRP were signiﬁcantly increased in overweight/obese children compared to normal weight. This study aimed to evaluate the association of AMY1 copy number variant (CNV) with obesity and inflammatory markers. Seventy-six participants aged between 6 and 10 years have participated, and the saliva samples were collected along with the anthropometric measurements.

Methods: AMY1 copy number was analyzed by 3D digital PCR, and obesity-related biomarkers were performed with a Bioplex multiplex analyzer.

Results: The mean AMY1 copy number was higher in normal weight (7.90 ± 0.38) compared to the overweight/obese group (6.20 ± 0.29). The association of AMY1 CNV with obesity and inflammatory markers showed significant negative correlation [CRP, β = -0.238 (p < 0.05); resistin, β = -0.25 (p < 0.05); MCP-1, β = -0.304 (p < 0.01)] except for complement factor D, TNF α and IL-6. The anti-inflammatory cytokine, IL-10 reported a positive correlation with AMY1 copy number with a β = 0.268 (p < 0.05). The multivariable model adjusted with age and gender depicted a similar correlation with obesity markers.

Conclusion: Our results report that AMY1 CNV is associated with obesity and inflammatory biomarkers in children's saliva sample.

Keywords: AMY1 copy numbers; body mass index; childhood obesity; inflammatory markers; obesity-related markers.

Grants and funding

This work was supported by the Alabama Agricultural Experiment Station and the Hatch Program of the National Institute of Food and Agriculture, US Department of Agriculture to J.R.B. and T.G.