Altered mitochondrial proteome and functional dynamics in patients with rheumatoid arthritis

Abstract

The autoimmune inflammatory disease, Rheumatoid arthritis (RA), has known imbalances in energy metabolism and superoxide levels thus may have an etiology associated with mitochondrial dysfunction. We thus evaluated the presence of a differential mitochondrial proteome as well as other characteristics including mitochondrial mass, membrane potential (Ψm), total cellular ATP and superoxide levels. Eighteen mitochondrial proteins were down-regulated while four were up-regulated in RA patients in comparison to the healthy controls (HC). A significant decrease in mitochondrial Ψm, superoxides and cellular ATP levels was observed in RA with constant mitochondrial mass suggesting mitochondrial dysfunction responsible for functional disparity in RA.

Keywords: Isobaric tags for relative and absolute quantitation employed with two-dimensional liquid chromatography–tandem mass spectrometry (iTRAQ-LC–MS/MS); Mitochondrial dysfunction; Mitochondrial membrane potential; Oxidative stress; Rheumatoid arthritis.