Genome-wide identification and expression analysis of jasmonate ZIM domain gene family in tuber mustard (Brassica juncea var. tumida)

PLoS One. 2020 Jun 16;15(6):e0234738. doi: 10.1371/journal.pone.0234738. eCollection 2020.

Abstract

Tuber mustard, which is the raw material of Fuling pickle, is a crop with great economic value. However, during growth and development, tuber mustard is frequently attacked by the pathogen Plasmodiophora brassicae and frequently experiences salinity stress. Jasmonic acid (JA) is a hormone related to plant resistance to biotic and abiotic stress. Jasmonate ZIM domain proteins (JAZs) are crucial components of the JA signaling pathway and play important roles in plant responses to biotic and abiotic stress. To date, no information is available about the characteristics of the JAZ family genes in tuber mustard. Here, 38 BjJAZ genes were identified in the whole genome of tuber mustard. The BjJAZ genes are located on 17 of 18 chromosomes in the tuber mustard genome. The gene structures and protein motifs of the BjJAZ genes are conserved between tuber mustard and Arabidopsis. The results of qRT-PCR analysis showed that BjuA030800 was specifically expressed in root, and BjuA007483 was specifically expressed in leaf. In addition, 13 BjJAZ genes were transiently induced by P. brassicae at 12 h, and 7 BjJAZ genes were induced by salt stress from 12 to 24 h. These results provide valuable information for further studies on the role of BjJAZ genes in the regulation of plant growth and development and in the response to biotic and abiotic stress.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Arabidopsis / genetics
  • Arabidopsis / metabolism
  • Cyclopentanes / metabolism
  • Gene Expression Regulation, Plant
  • Genome, Plant*
  • Multigene Family
  • Mustard Plant / genetics*
  • Mustard Plant / metabolism
  • Oxylipins / metabolism
  • Phylogeny
  • Plant Leaves / metabolism
  • Plant Proteins / classification
  • Plant Proteins / genetics*
  • Plant Proteins / metabolism
  • Plant Roots / metabolism
  • Protein Domains
  • RNA, Plant / metabolism
  • Repressor Proteins / classification
  • Repressor Proteins / genetics*
  • Repressor Proteins / metabolism
  • Stress, Physiological

Substances

  • Cyclopentanes
  • Oxylipins
  • Plant Proteins
  • RNA, Plant
  • Repressor Proteins
  • jasmonic acid

Grants and funding

This work is supported by the National Natural Science Foundation of China (Program Nos. 31701451, 31770407 and 31800311), Zhaoming Cai, Diandong Wang and Jingjing Liao; the Chongqing Basic Research and Frontier Exploration Project (Program No.cstc2019jcyj-msxmX0285), Zhaoming Cai; the Science and Technology Research Program of Chongqing Municipal Education Commission (Grant No. KJQN201901406), Zhaoming Cai. The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript.