Evaluation of antioxidant capacity and immunomodulatory effects of yeast hydrolysates for hepatocytes of blunt snout bream (Megalobrama amblycephala)

Samad Rahimnejad; Xiang-Yang Yuan; Wen-Bin Liu; Guang-Zhen Jiang; Xiu-Fei Cao; Yong-Jun Dai; Cong-Cong Wang; Hesham Eed Desouky

doi:10.1016/j.fsi.2020.06.019

Evaluation of antioxidant capacity and immunomodulatory effects of yeast hydrolysates for hepatocytes of blunt snout bream (Megalobrama amblycephala)

Fish Shellfish Immunol. 2020 Nov:106:142-148. doi: 10.1016/j.fsi.2020.06.019. Epub 2020 Jun 12.

Authors

Samad Rahimnejad¹, Xiang-Yang Yuan², Wen-Bin Liu³, Guang-Zhen Jiang³, Xiu-Fei Cao⁴, Yong-Jun Dai³, Cong-Cong Wang³, Hesham Eed Desouky³

Affiliations

¹ University of South Bohemia in Ceske Budejovice, Faculty of Fisheries and Protection of Waters, South Bohemian Research Center of Aquaculture and Biodiversity of Hydrocenoses, Zatisi 728/ II, 389 25, Vodnany, Czech Republic.
² Jiangsu Provincial Key Construction Laboratory of Probiotics Preparation, School of Life Science and Food Engineering, Huaiyin Institute of Technology, Huaian, 223003, PR China. Electronic address: Xiangyangyuan007@hyit.edu.cn.
³ National Experimental Teaching Demonstration Center of Animal Science, College of Animal Science and Technology, Nanjing Agricultural University, Nanjing, 210095, PR China.
⁴ The Key Laboratory of Aquaculture Nutrition and Feed, Ministry of Agriculture and Rural Affairs, The Key Laboratory of Mariculture, Ministry of Education, Fisheries College, Ocean University of China, Qingdao, 266003, PR China.

PMID: 32540503
DOI: 10.1016/j.fsi.2020.06.019

Abstract

An in-vitro study was carried out to examine the effects of yeast hydrolysate (YH) on antioxidant capacity and innate immunity of blunt snout bream (Megalobrama amblycephala) hepatocytes. Fish primary hepatocytes were seeded at a density of 3 × 10⁵ cells mL^-1 in 6-well tissue culture plates and treated with two different media including: 1) DMEM/F12 medium (control), and 2) YH medium [DMEM/F12 + 0.1 g L^-1 YH]. After incubation for 24 h, the culture medium and primary hepatocytes were collected for subsequent analyses. The results showed no significant (P > 0.05) effect of YH on aspartate aminotransferase (AST), alanine aminotransferase (ALT) and lactate dehydrogenase (LDH) activities and urea nitrogen (UN) concentration in the conditioned medium. However, significantly (P < 0.05) higher ALT and AST activities were found in YH treated hepatocytes compared to control. Moreover, YH supplementation led to significant enhancement of superoxide dismutase (SOD), catalase (CAT), alternative complement pathway (ACH50) and glutathione peroxidase (GPX) activities and reduction of malondialdehyde (MDA) concentration in the conditioned medium. Furthermore, YH application upregulated the expression of SOD, CAT and NOX2 genes and downregulated mRNA levels of Keap1, Nrf2 and Bach1 in hepatocytes. Also, markedly higher lysozyme activity and albumin concentration were found in the conditioned medium of YH group compared to the control. Additionally, expression of immune-related genes such as antimicrobial peptides 1 (Leap 1) and Leap 2 were significantly upregulated by YH application. Down-regulated expression of NADPH oxidase-2 (NOX2), Kelch-like-ECH-associated protein 1 (Keap1), NF-E2-related factor 2 (Nrf2) and BTB and CNC homolog 1 (Bach1) were observed in YH treated hepatocytes. To conclude, YH supplementation improved antioxidant capacity and innate immunity of blunt snout bream hepatocytes.

Keywords: Antioxidant activity; Blunt snout bream; Hepatocyte; Innate immunity; Yeast hydrolysate.

MeSH terms

Animals
Antioxidants / metabolism*
Cyprinidae / immunology*
Hepatocytes / drug effects
Hepatocytes / immunology*
Immunomodulation / drug effects*
Protein Hydrolysates / pharmacology*
Yeast, Dried / pharmacology*

Substances

Antioxidants
Protein Hydrolysates