Urinary autotaxin concentrations are associated with kidney injury

Yoshifumi Morita; Makoto Kurano; Eriko Morita; Satoshi Shimamoto; Koji Igarashi; Motoji Sawabe; Junken Aoki; Yutaka Yatomi

doi:10.1016/j.cca.2020.06.019

Urinary autotaxin concentrations are associated with kidney injury

Clin Chim Acta. 2020 Oct:509:156-165. doi: 10.1016/j.cca.2020.06.019. Epub 2020 Jun 12.

Authors

Yoshifumi Morita¹, Makoto Kurano², Eriko Morita³, Satoshi Shimamoto⁴, Koji Igarashi⁴, Motoji Sawabe⁵, Junken Aoki⁶, Yutaka Yatomi⁷

Affiliations

¹ Department of Clinical Laboratory, the University of Tokyo Hospital, Tokyo, Japan; Department of Molecular Pathology, Graduate School of Medical and Dental Sciences, Tokyo Medical and Dental University, Tokyo, Japan.
² Department of Clinical Laboratory, the University of Tokyo Hospital, Tokyo, Japan; Department of Clinical Laboratory Medicine, Graduate School of Medicine, the University of Tokyo, Tokyo, Japan. Electronic address: kurano-tky@umin.ac.jp.
³ Department of Clinical Laboratory, the University of Tokyo Hospital, Tokyo, Japan.
⁴ Bioscience Division, TOSOH Corporation, Kanagawa, Japan.
⁵ Department of Molecular Pathology, Graduate School of Medical and Dental Sciences, Tokyo Medical and Dental University, Tokyo, Japan.
⁶ Laboratory of Molecular and Cellular Biochemistry, Graduate School of Pharmaceutical Sciences, Tohoku University, Miyagi, Japan.
⁷ Department of Clinical Laboratory, the University of Tokyo Hospital, Tokyo, Japan; Department of Clinical Laboratory Medicine, Graduate School of Medicine, the University of Tokyo, Tokyo, Japan.

PMID: 32540127
DOI: 10.1016/j.cca.2020.06.019

Abstract

Background: While basic researches have shown the involvement of the autotaxin-lysophosphatidic acid (ATX-LPA) axis in the pathogenesis of kidney diseases, no clinical studies have revealed the association between urinary ATX concentrations and kidney disease yet. We investigate the clinical characteristics in relation to the urinary ATX concentrations and the potential association between urinary ATX concentrations and various kidney diseases.

Methods: We measured the urinary ATX concentrations in residual urine samples after routine clinical testing from a total of 326 subjects with various kidney diseases and healthy subjects. We compared the urinary ATX concentrations in relation to clinical parameters and urinary biomarkers, and investigated their association with various kidney diseases.

Results: The urinary ATX concentrations were associated with the gender, eGFR, presence/absence of hematuria, serum ATX, urinary concentrations of total protein (TP), microalbumin, N-acetyl-β-D-glucosaminidase (NAG), α1-microglobulin (α1-MG), and transforming growth factor-β. Multiple regression analyses identified urinary α1-MG, age, urinary TP, NAG, and hematuria as being significantly associated with the urinary ATX concentrations. Urinary ATX concentrations were higher in subjects with membranous nephropathy and systemic lupus erythematosus than in the control subjects.

Conclusions: Urinary ATX might be associated with pathological conditions of the kidney associated with kidney injury.

Keywords: Autotaxin; Kidney disease; Nephritis; Urine.

MeSH terms

Biomarkers
Glomerular Filtration Rate
Humans
Kidney*
Phosphoric Diester Hydrolases*
Transforming Growth Factor beta

Substances

Biomarkers
Transforming Growth Factor beta
Phosphoric Diester Hydrolases