Influence of chitosan average molecular weight on degradation and stability of electrodeposited conduits

Katarzyna Nawrotek; Michał Tylman; Agnieszka Adamus-Włodarczyk; Karolina Rudnicka; Justyna Gatkowska; Marek Wieczorek; Radosław Wach

doi:10.1016/j.carbpol.2020.116484

Influence of chitosan average molecular weight on degradation and stability of electrodeposited conduits

Carbohydr Polym. 2020 Sep 15:244:116484. doi: 10.1016/j.carbpol.2020.116484. Epub 2020 May 29.

Authors

Katarzyna Nawrotek¹, Michał Tylman², Agnieszka Adamus-Włodarczyk³, Karolina Rudnicka⁴, Justyna Gatkowska⁵, Marek Wieczorek⁶, Radosław Wach³

Affiliations

¹ Department of Process Thermodynamics, Faculty of Process and Environmental Engineering, Lodz University of Technology, Wolczanska 213 Street, 90-924, Lodz, Poland. Electronic address: katarzyna.nawrotek@p.lodz.pl.
² Department of Process Thermodynamics, Faculty of Process and Environmental Engineering, Lodz University of Technology, Wolczanska 213 Street, 90-924, Lodz, Poland.
³ Institute of Applied Radiation Chemistry, Faculty of Chemistry, Lodz University of Technology, Wroblewskiego 15 Street, 93-590, Lodz, Poland.
⁴ Department of Immunology and Infectious Biology, Faculty of Biology and Environmental Protection, University of Lodz, Banacha 12/16 Street, 90-237, Lodz, Poland.
⁵ Department of Immunoparasitology, Faculty of Biology and Environmental Protection, University of Lodz, Banacha 12/16 Street, 90-237, Lodz, Poland.
⁶ Department of Neurobiology, Faculty of Biology and Environmental Protection, University of Lodz, Pomorska 141/143 Street, 90-236, Lodz, Poland.

PMID: 32536389
DOI: 10.1016/j.carbpol.2020.116484

Abstract

Tubular chitosan-based hydrogels, obtained in an electrodeposition process, are subject of degradation and stability studies. The implants are prepared from polymer with different average molecular weight. This approach allows fabricating structures that vary in mass and wall thickness. The obtained implants are incubated in phosphate buffered solution (pH 7.4) with or without lysozyme up to 56 days at 37 °C. Subsequently, chemical, physical as well as mechanical properties of implants are evaluated. Although the initial physicomechanical properties are different, they change upon incubation and remain similar over its period. Finally, in vitro biocompatibility of implants is proven after assessing their action towards mHippoE-18 embryonic hippocampal cells and THP1-XBlue™ monocytes. Since dimensions of nerves and the gap length differ across the body and injury, respectively, the possibility to control properties of chitosan applied gives a tool to prepare implants with wall thickness adjusted to the specific peripheral nerve injury case.

Keywords: Chitosan; Conduit; Degradation; Electrodeposition; Hydrogel; Peripheral nerve regeneration.