Molecular characterization of an uncommon multigene Reassortant G1P[4] rotavirus identified in China

Yuanjun Zeng; Biyan Zhao; Tingdong Li; Shiyin Zhang; Yingbin Wang; Hongmei Xu; Shengxiang Ge; Ningshao Xia

doi:10.1016/j.meegid.2020.104413

Molecular characterization of an uncommon multigene Reassortant G1P[4] rotavirus identified in China

Infect Genet Evol. 2020 Nov:85:104413. doi: 10.1016/j.meegid.2020.104413. Epub 2020 Jun 11.

Authors

Yuanjun Zeng¹, Biyan Zhao¹, Tingdong Li², Shiyin Zhang¹, Yingbin Wang¹, Hongmei Xu³, Shengxiang Ge⁴, Ningshao Xia⁵

Affiliations

¹ State Key Laboratory of Molecular Vaccinology and Molecular Diagnostics, National Institute of Diagnostics and Vaccine Development in Infectious Disease, Collaborative Innovation Center of Biological Products, School of public health, Xiamen university, Xiamen, China.
² State Key Laboratory of Molecular Vaccinology and Molecular Diagnostics, National Institute of Diagnostics and Vaccine Development in Infectious Disease, Collaborative Innovation Center of Biological Products, School of public health, Xiamen university, Xiamen, China. Electronic address: litingdong@xmu.edu.cn.
³ Children's Hospital of Chongqing Medical University, Ministry of Education Key Laboratory of Child Development and Disorders, China International Science and Technology Cooperation Base of Child Development, Critical Disorders, Chongqing Key Laboratory of Pediatrics, Chongqing 400014, China.
⁴ State Key Laboratory of Molecular Vaccinology and Molecular Diagnostics, National Institute of Diagnostics and Vaccine Development in Infectious Disease, Collaborative Innovation Center of Biological Products, School of public health, Xiamen university, Xiamen, China. Electronic address: sxge@xmu.edu.cn.
⁵ State Key Laboratory of Molecular Vaccinology and Molecular Diagnostics, National Institute of Diagnostics and Vaccine Development in Infectious Disease, Collaborative Innovation Center of Biological Products, School of public health, Xiamen university, Xiamen, China; The Research Unit of Frontier Technology of Structural Vaccinology, Chinese Academy of Medical Sciences, Beijing 100730, China.

PMID: 32535160
DOI: 10.1016/j.meegid.2020.104413

Abstract

Rotavirus A (RVA) is the leading cause of acute gastroenteritis worldwide in both young children and animals. In this study, we analyzed the complete genome sequence of a rare G1P[4] rotavirus, which was identified from the stool samples (20130113) of an infant with diarrhea in Chongqing, China. The genotype constellation of strain 20130113 was G1-P[4]-I2-R1-C1-M1-A2-N2-T1-E1-H1. The results of both BLAST and phylogenic analysis indicated that 20130113 was likely generated by genetic reassortment between G1P[8] and G2P[4] strains that co-circulated during the season. Whether the emergence of this uncommon genotype reflects the establishment of a new RVA strain in the population requires continuous monitoring of rotavirus epidemiology.

Keywords: G1P[4]; Genotype constellation; Intergenogroup Reassortant; RVA.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

China
Diarrhea / virology
Evolution, Molecular*
Feces / virology
Gastroenteritis / virology*
Genetic Variation
Genome, Viral
Genotype
Humans
Infant
Phylogeny
RNA, Viral
Reassortant Viruses / classification*
Reassortant Viruses / genetics*
Reassortant Viruses / isolation & purification
Rotavirus / classification*
Rotavirus / genetics*
Rotavirus / isolation & purification
Rotavirus Infections / virology*
Sequence Analysis, DNA
Whole Genome Sequencing

Substances

RNA, Viral