Association of a genetic variant in the angiopoietin-like protein 4 gene with cervical cancer

Farzad Rahmani; Malihe Hasanzadeh; Seyed Mahdi Hassanian; Majid Khazaei; Habibollah Esmaily; Seyed Ami Asef-Agah; Alireza Naghipour; Gordon A Ferns; Amir Avan

doi:10.1016/j.prp.2020.153011

Association of a genetic variant in the angiopoietin-like protein 4 gene with cervical cancer

Pathol Res Pract. 2020 Jul;216(7):153011. doi: 10.1016/j.prp.2020.153011. Epub 2020 May 15.

Authors

Farzad Rahmani¹, Malihe Hasanzadeh², Seyed Mahdi Hassanian³, Majid Khazaei⁴, Habibollah Esmaily⁵, Seyed Ami Asef-Agah⁶, Alireza Naghipour⁶, Gordon A Ferns⁷, Amir Avan⁸

Affiliations

¹ Iranshahr University of Medical Sciences, Iranshahr, Iran; Student Research Committee, Faculty of Medicine, Mashhad University of Medical Sciences, Mashhad, Iran.
² Department of Gynecology Oncology, Woman Health Research Center, Faculty of Medicine, Mashhad University of Medical Sciences, Mashhad, Iran.
³ Metabolic Syndrome Research Center, Mashhad University of Medical Sciences, Mashhad, Iran; Student Research Committee, Faculty of Medicine, Mashhad University of Medical Sciences, Mashhad, Iran.
⁴ Metabolic Syndrome Research Center, Mashhad University of Medical Sciences, Mashhad, Iran.
⁵ Department of Epidemiology and Biostatistics, School of Health, Mashhad University of Medical Sciences, Mashhad, Iran.
⁶ Student Research Committee, Faculty of Medicine, Mashhad University of Medical Sciences, Mashhad, Iran.
⁷ Brighton & Sussex Medical School, Division of Medical Education, Falmer, Brighton, Sussex BN1 9PH, UK.
⁸ Metabolic Syndrome Research Center, Mashhad University of Medical Sciences, Mashhad, Iran; Student Research Committee, Faculty of Medicine, Mashhad University of Medical Sciences, Mashhad, Iran. Electronic address: avana@mums.ac.ir.

PMID: 32534714
DOI: 10.1016/j.prp.2020.153011

Abstract

Background: Cervical cancer is among the most aggressive gynecological tumors and is a consequence of interactions between genetic and epigenetic factors. Several genetic polymorphisms related to cervical cancer have been reported in previous clinical studies. In this study, we aimed to explore the possible relationship between polymorphisms of the ANGPTL4 gene locus and susceptibility to cervical cancer.

Methods: We investigated the relationship between a single nucleotide polymorphism (SNP) in the ANPGTL4 gene (rs116843064) and risk of cervical cancer in a total of 378 individuals with (n = 151), or without (n = 227) cancer. DNA was extracted, and genotyped using a Taq-Man based real time PCR.

Results: The ANPGTL4 polymorphism was found to be associated with an increased risk of developing cervical neoplasia using dominant model (OR = 12.48, CI = 4.9-31.82, p < 0.0001) and additive model (OR = 30.54, CI = 7.35-126.89, p < 0.0001).

Conclusion: Our results indicate that there is a strong association between ANPGTL4 and the susceptibility for cervical cancer suggesting that it is a potential risk factor for cervical neoplasia.

Keywords: ANGPTL4; Cervical neoplasia; Polymorphism.

MeSH terms

Adult
Aged
Angiopoietin-Like Protein 4 / genetics*
Female
Genetic Predisposition to Disease / genetics*
Genotype
Humans
Middle Aged
Polymorphism, Single Nucleotide
Uterine Cervical Neoplasms / genetics*

Substances

ANGPTL4 protein, human
Angiopoietin-Like Protein 4