Fungal biosynthesis of lignin-modifying enzymes from pulp wash and Luffa cylindrica for azo dye RB5 biodecolorization using modeling by response surface methodology and artificial neural network

Clara Dourado Fernandes; Victor Ruan Silva Nascimento; Diego Batista Meneses; Débora S Vilar; Nádia Hortense Torres; Manuela Souza Leite; José Roberto Vega Baudrit; Muhammad Bilal; Hafiz M N Iqbal; Ram Naresh Bharagava; Silvia Maria Egues; Luiz Fernando Romanholo Ferreira

doi:10.1016/j.jhazmat.2020.123094

Fungal biosynthesis of lignin-modifying enzymes from pulp wash and Luffa cylindrica for azo dye RB5 biodecolorization using modeling by response surface methodology and artificial neural network

J Hazard Mater. 2020 Nov 15:399:123094. doi: 10.1016/j.jhazmat.2020.123094. Epub 2020 Jun 4.

Affiliations

¹ Graduate Program in Process Engineering, Tiradentes University, Murilo Dantas Avenue, 300, Farolândia, 49032-490, Aracaju, Sergipe, Brazil. Electronic address: claraa.fernandees@gmail.com.
² Graduate Program in Process Engineering, Tiradentes University, Murilo Dantas Avenue, 300, Farolândia, 49032-490, Aracaju, Sergipe, Brazil.
³ National Nanotechnology Laboratory, National Center for High Technology, 10109, Pavas, San José, Costa Rica.
⁴ Graduate Program in Process Engineering, Tiradentes University, Murilo Dantas Avenue, 300, Farolândia, 49032-490, Aracaju, Sergipe, Brazil; Institute of Technology and Research, Murilo Dantas Avenue, 300, Farolândia, 49032-490, Aracaju, Sergipe, Brazil. Electronic address: nadiahortense@gmail.com.
⁵ Graduate Program in Process Engineering, Tiradentes University, Murilo Dantas Avenue, 300, Farolândia, 49032-490, Aracaju, Sergipe, Brazil; Institute of Technology and Research, Murilo Dantas Avenue, 300, Farolândia, 49032-490, Aracaju, Sergipe, Brazil.
⁶ National Nanotechnology Laboratory, National Center for High Technology, 10109, Pavas, San José, Costa Rica; Laboratory of Polymer Science and Technology, School of Chemistry, Universidad Nacional, Campus Omar Dengo, 86-3000, Heredia, Costa Rica.
⁷ School of Life Science and Food Engineering, Huaiyin Institute of Technology, Huaian, China.
⁸ Tecnologico de Monterrey, School of Engineering and Sciences, Campus Monterrey, Ave. Eugenio Garza Sada 2501, Monterrey, N.L., CP 64849, Mexico.
⁹ Laboratory for Bioremediation and Metagenomics Research (LBMR), Department of Microbiology (DM), Babasaheb Bhimrao Ambedkar University (A Central University), Vidya Vihar, Raebareli Road, Lucknow, 226 025, Uttar Pradesh, India.
¹⁰ Graduate Program in Process Engineering, Tiradentes University, Murilo Dantas Avenue, 300, Farolândia, 49032-490, Aracaju, Sergipe, Brazil; Institute of Technology and Research, Murilo Dantas Avenue, 300, Farolândia, 49032-490, Aracaju, Sergipe, Brazil. Electronic address: romanholobio@gmail.com.

PMID: 32534401
DOI: 10.1016/j.jhazmat.2020.123094

Abstract

This study demonstrates the evaluation between the artificial neural network technique coupled to the genetic algorithm (ANN-GA) and the response surface methodology (RSM) for prediction of Reactive Black 5 (RB5) decolorization by crude enzyme from Pleurotus. sajor-caju. Fungal lignin-modifying enzymes (FLME) were synthesized using pulp wash (PW) as an inducing substrate, and L. cylindrica (L.C) for cell immobilization. When grown in PW, the fungus showed higher Lac activity (126.5 IU. mL^-1), whereas when immobilized a higher MnP activity was achieved (22.79 IU. mL^-1), but both methods were capable of decolorizing the dye in about 89.4 % and 75 %, respectively. This indicates applicability of PW as an alternative substrate for FLME induction and viability of immobilization for MnP synthesis. For RB5 decolorization, the action of the crude enzyme extract was considered as a function of pH, dye concentration, temperature, and reaction time. The models are well adjusted to predict the efficiency of biodecolorization, with no statistical difference between ANN-GA and RSM, which indicates potential for green enzymes prospecting application in bioprocess industry.

Keywords: Genetic algorithm; Green enzymes; Immobilization; Pleurotus sajor-caju; Reactive Black 5.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Azo Compounds*
Lignin
Luffa*
Naphthalenesulfonates
Neural Networks, Computer

Substances

Azo Compounds
Naphthalenesulfonates
Lignin
Remazol black B