Injectable and bioactive methylcellulose hydrogel carrying bone mesenchymal stem cells as a filler for critical-size defects with enhanced bone regeneration

Lizhi Deng; Yun Liu; Liqun Yang; Ju-Zhen Yi; Feilong Deng; Li-Ming Zhang

doi:10.1016/j.colsurfb.2020.111159

Injectable and bioactive methylcellulose hydrogel carrying bone mesenchymal stem cells as a filler for critical-size defects with enhanced bone regeneration

Colloids Surf B Biointerfaces. 2020 Oct:194:111159. doi: 10.1016/j.colsurfb.2020.111159. Epub 2020 May 31.

Authors

Lizhi Deng¹, Yun Liu², Liqun Yang¹, Ju-Zhen Yi¹, Feilong Deng³, Li-Ming Zhang⁴

Affiliations

¹ DSAPM Lab and PCFM Lab, School of Materials Science and Engineering, School of Chemistry, Sun Yat-sen University, Guangzhou, 510275, China.
² Department of Oral Implantology, Guanghua School of Stomatology, Sun Yat-sen University, Guangzhou, 510055, China.
³ Department of Oral Implantology, Guanghua School of Stomatology, Sun Yat-sen University, Guangzhou, 510055, China. Electronic address: dengfl@mail.sysu.edu.cn.
⁴ DSAPM Lab and PCFM Lab, School of Materials Science and Engineering, School of Chemistry, Sun Yat-sen University, Guangzhou, 510275, China. Electronic address: ceszhlm@mail.sysu.edu.cn.

PMID: 32534367
DOI: 10.1016/j.colsurfb.2020.111159

Abstract

As a soluble cellulose derivative, methylcellulose (MC), can be used to construct thermosensitive hydrogels. However, a pure MC gel is generally considered an inert material that is inadequate for cell survival. We adopted an environmentally friendly method to fabricate a nano-hydroxyapatite (nHA) hybrid MC hydrogel. Rheology results showed that the addition of nHA increased the gelation temperature. Furthermore, the live/dead assay confirmed that the addition of nHA improved the survival of bone marrow-derived mesenchymal stem cells (BMSCs) inside the gel. In addition, ARS staining indicated that the presence of nHA stimulated osteogenic differentiation. Finally, in vivo cranial defect experiments showed improved remediation efficiency when using the nHA hybrid MC hydrogel to carry BMSCs.

Keywords: BMSCs; Bone regeneration; Hydrogel; Methylcellulose; nHA.

MeSH terms

Bone Regeneration*
Hydrogels
Mesenchymal Stem Cells*
Methylcellulose*
Osteogenesis
Tissue Scaffolds*

Substances

Hydrogels
Methylcellulose