MicroRNA-155 complementation on a chemically functionalized dual electrode surface for determining breast cancer

Subash C B Gopinath; Veeradasan Perumal; Shijin Xuan

doi:10.1007/s13205-020-02261-x

MicroRNA-155 complementation on a chemically functionalized dual electrode surface for determining breast cancer

3 Biotech. 2020 Jun;10(6):270. doi: 10.1007/s13205-020-02261-x. Epub 2020 May 28.

Authors

Subash C B Gopinath^{1

2}, Veeradasan Perumal³, Shijin Xuan⁴

Affiliations

¹ School of Bioprocess Engineering, Universiti Malaysia Perlis, 02600 Arau, Perlis Malaysia.
² Institute of Nano Electronic Engineering, Universiti Malaysia Perlis, 01000 Kangar, Perlis Malaysia.
³ Mechanical Engineering Department, Universiti Teknologi PETRONAS, 32610 Seri Iskandar, Perak Darul Ridzuan Malaysia.
⁴ Department of Mammary and Thyroid Surgery, Central Hospital Affiliated to Shandong First Medical University, No. 105 Jiefang Road, Lixia District, 250013 Jinan, Shandong Province People's Republic of China.

Abstract

This study correlated and quantified the expression of microRNA-155 with breast cancer to determine breast cancer progression. The target microRNA-155 sequence was identified by complementation on a capture-probe sequence-immobilized interdigitated dual electrode surface. The sensitivity was found to be 1 fM, and the limit of detection fell between 1 and 10 fM. The specific sequence selectivity with single mismatches, triple mismatches, and noncomplementary bases failed to complement the capture-probe sequence. The obtained results demonstrate the selective determination of the microRNA-155 sequence and can help to diagnose breast cancer.

Keywords: Biosensor; Breast cancer; Interdigitated electrode; Surface chemistry; microRNA-155.