Oral intake of lipopolysaccharide regulates toll-like receptor 4-dependent granulopoiesis

Exp Biol Med (Maywood). 2020 Aug;245(14):1254-1259. doi: 10.1177/1535370220931043. Epub 2020 Jun 9.

Abstract

In our present study, we investigated the impact of LPS on neutrophil homeostasis and found that oral intake is sufficient to induce hematopoietic stem and progenitor cell fate decisions towards the neutrophil lineage independent of G-CSF. In addition, TLR4 has been identified as the indispensable sensor for oral LPS-modulated steady-state granulopoiesis. We provide evidence that the gastrointestinal microbiome is critical for neutrophil homeostasis, which has implications for patients being treated with chemotherapy or antimicrobial therapy, since both are significantly influencing the composition of the intestinal microbiome.

Keywords: G-CSF; Lipopolysaccharide; TLR4; granulocytes; granulopoiesis.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Administration, Oral
  • Animals
  • Gastrointestinal Microbiome / drug effects
  • Granulocyte Colony-Stimulating Factor / metabolism
  • Granulocytes / drug effects*
  • Granulocytes / metabolism*
  • Hematopoietic Stem Cells / drug effects
  • Hematopoietic Stem Cells / metabolism
  • Lipopolysaccharides / administration & dosage*
  • Mice
  • Mice, Inbred C57BL
  • Neutropenia / drug therapy
  • Neutropenia / metabolism
  • Neutrophils / drug effects
  • Neutrophils / metabolism
  • Rhodobacter sphaeroides / metabolism
  • Stem Cells / drug effects
  • Stem Cells / metabolism
  • Toll-Like Receptor 4 / metabolism*

Substances

  • Lipopolysaccharides
  • Tlr4 protein, mouse
  • Toll-Like Receptor 4
  • Granulocyte Colony-Stimulating Factor