The aim was to find out the impact of stress, caused by agrotechnological tools on photosynthetic behaviour of apple trees. The apple tree (Malus domestica Borkh.) cultivar Rubin was grafted on dwarfing rootstocks P60, planted in single rows spaced 1.25 m apart with 3.5 m between rows. In contrast to plant senescing reflectance index and nitrogen balance index, the photochemical reflectance index was significantly higher in 2018 compared with 2017. Such differences might be caused by drought stress on the summer and fast recovery before harvest time when measurements were made. The movement of nutrients and water disrupted by trunk incision had significantly negative effect on reflectance indices regardless on the year. Mechanical pruning with trunk incision and calcium-prohexadione lead to decreased dry to fresh weight ratio by 10-12% in first year of treatment. Mechanical pruning had significantly negative impact on photosynthetic rate, compared to pruning by super spindle it decreased 47%. Strong positive correlation between PRI and NBI 0,89-0,94, and strong negative correlations between PRI, NBI and PSRI -0.88 - (-0.91) were determined.