Hypoxia promotes proliferation of pituitary adenomas by HIF-1α/ALKBH5 signaling in vitro

Yuan Qian; Chao Zhang; Wei Wang; Di Lu; Junjun Li; Liyan Li; Yao Li; Yisheng Qiao; Hao Song; Xingli Deng

Hypoxia promotes proliferation of pituitary adenomas by HIF-1α/ALKBH5 signaling in vitro

Int J Clin Exp Pathol. 2020 May 1;13(5):1030-1034. eCollection 2020.

Authors

Yuan Qian^{1

2}, Chao Zhang³, Wei Wang^{3

4}, Di Lu⁵, Junjun Li³, Liyan Li⁶, Yao Li³, Yisheng Qiao³, Hao Song³, Xingli Deng³

Affiliations

¹ Yunnan Key Laboratory of Laboratory Medicine, 1st Affiliated Hospital of Kunming Medical University Kunming, China.
² Department of Medical Genetics and Prenatal Diagnosis, Kunming Maternal and Child Health Hospital Kunming, China.
³ Department of Neurosurgery, 1st Affiliated Hospital of Kunming Medical University Kunming, Yunnan Province, China.
⁴ Department of Neurosurgery, The People's Hospital of Chuxiong Chuxiong, Yunnan Province, China.
⁵ Biomedical Engineering Research Center, Kunming Medical University Kunming, Yunnan Province, China.
⁶ Institute of Neuroscience, Kunming Medical University Kunming, Yunnan Province, China.

PMID: 32509075
PMCID: PMC7270662

Abstract

Hypoxia is a common phenomenon in pituitary adenomas (PAs). The role and mechanism of hypoxia in the PAs remains elusive. This work aimed to explore the effect of hypoxia on PAs in vitro. PA cells GT1-1 were cultured and treated under hypoxic condition. Cell proliferation assay showed the proliferation of PA cells was increased significantly by hypoxia treatment, with a peak at 12 hours. qPCR and western blot indicated that the expression of HIF-1α, ALKBH5, and Nanog were elevated by hypoxia stimuli. In conclusion, our funding demonstrated that hypoxia could increase Nanog expression through HIF-1α/ALKBH5 signaling, thereby promoting the proliferation of PA cells.

Keywords: ALKBH5; HIF-1α; Nanog; Pituitary adenoma; hypoxia.