The AtGSTU7 gene influences glutathione-dependent seed germination under ABA and osmotic stress in Arabidopsis

Juan Wu; Nan Zhang; Ziguang Liu; Shengyi Liu; Chunxiao Liu; Jianhui Lin; He Yang; Shuang Li; Yasushi Yukawa

doi:10.1016/j.bbrc.2020.05.153

The AtGSTU7 gene influences glutathione-dependent seed germination under ABA and osmotic stress in Arabidopsis

Biochem Biophys Res Commun. 2020 Jul 30;528(3):538-544. doi: 10.1016/j.bbrc.2020.05.153. Epub 2020 Jun 4.

Authors

Juan Wu¹, Nan Zhang², Ziguang Liu³, Shengyi Liu⁴, Chunxiao Liu⁵, Jianhui Lin², He Yang², Shuang Li⁶, Yasushi Yukawa⁷

Affiliations

¹ Key Laboratory of Saline-alkali Vegetation Ecology Restoration, Ministry of Education, College of Life Sciences, Northeast Forestry University, Harbin, 150040, China. Electronic address: wuj1970@163.com.
² Key Laboratory of Saline-alkali Vegetation Ecology Restoration, Ministry of Education, College of Life Sciences, Northeast Forestry University, Harbin, 150040, China.
³ Key Laboratory of Combining Farming and Animal Husbandry, Institute of Animal Husbandry of Heilongjiang Academy of Agricultural Sciences, Ministry of Agriculture and Rural Affairs, PR, Harbin, 150040, China.
⁴ Mudanjiang Medical University, Mudanjiang, 157011, Heilongjiang, China.
⁵ State Key Laboratory for Molecular Biology of Special Economic Animals, Institute of Special Animal and Plant Sciences, Chinese Academy of Agricultural Sciences, Changchun, 130112, China.
⁶ Graduate School of Science, Nagoya City University, Nagoya, 467-8501, Japan.
⁷ Graduate School of Science, Nagoya City University, Nagoya, 467-8501, Japan. Electronic address: yyuk@nsc.nagoya-cu.ac.jp.

PMID: 32507596
DOI: 10.1016/j.bbrc.2020.05.153

Abstract

Glutathione S-transferases (GSTs) play important roles in metabolism and detoxification of plant cells. However, their functions during development are less well understood. Arabidopsis AtGSTU7 (AT2G29420) encodes a Tau class GST. Here we provide the AtGSTU7 was abundantly expressed in seeds and in roots at an early stage of germination. AtGSTU7 expression was repressed by exogenous ABA and promoted by osmotic stress. A null mutant of AtGSTU7 (atgstu7) accumulated higher contents of reduced GSH and decreased amounts of endogenous H₂O₂ in seedlings. The atgstu7 plants showed decreased osmotic tolerance during seed germination, which was influenced by GSH and ABI3 gene expression. The results suggested that AtGSTU7 involvement in seed germination is mediated by GSH-ROS homeostasis and ABA signaling.

Keywords: ABA signaling; Glutathione S-Transferases; Reactive oxygen species; Seed germination.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Abscisic Acid / metabolism
Abscisic Acid / pharmacology
Arabidopsis / genetics*
Arabidopsis / growth & development
Arabidopsis / metabolism*
Arabidopsis Proteins / antagonists & inhibitors
Arabidopsis Proteins / genetics*
Arabidopsis Proteins / metabolism
Gene Expression Regulation, Plant
Gene Knockout Techniques
Genes, Plant
Germination / genetics
Germination / physiology
Glutathione / metabolism
Glutathione Transferase / deficiency
Glutathione Transferase / genetics*
Glutathione Transferase / metabolism
Osmotic Pressure
Plant Growth Regulators / metabolism
Plant Growth Regulators / pharmacology
Plants, Genetically Modified
Reactive Oxygen Species / metabolism
Seeds / genetics
Seeds / growth & development
Seeds / metabolism
Signal Transduction
Transcription Factors / genetics
Transcription Factors / metabolism

Substances

ABI3 protein, Arabidopsis
Arabidopsis Proteins
Plant Growth Regulators
Reactive Oxygen Species
Transcription Factors
Abscisic Acid
Glutathione Transferase
Glutathione