LncRNA MIR31HG functions as a ceRNA to regulate c-Met function by sponging miR-34a in esophageal squamous cell carcinoma

Jie Chu; Jinlin Jia; Lijun Yang; Yunhui Qu; Huiqing Yin; Junhu Wan; Fucheng He

doi:10.1016/j.biopha.2020.110313

LncRNA MIR31HG functions as a ceRNA to regulate c-Met function by sponging miR-34a in esophageal squamous cell carcinoma

Biomed Pharmacother. 2020 Aug:128:110313. doi: 10.1016/j.biopha.2020.110313. Epub 2020 Jun 2.

Authors

Jie Chu¹, Jinlin Jia², Lijun Yang³, Yunhui Qu⁴, Huiqing Yin⁵, Junhu Wan⁶, Fucheng He⁷

Affiliations

¹ Department of Medical Laboratory, The First Affiliated Hospital, Zhengzhou University, Zhengzhou, Henan Province, China. Electronic address: 504017950@qq.com.
² Department of Medical Laboratory, The First Affiliated Hospital, Zhengzhou University, Zhengzhou, Henan Province, China. Electronic address: 2632943581@qq.com.
³ Department of Medical Laboratory, The First Affiliated Hospital, Zhengzhou University, Zhengzhou, Henan Province, China. Electronic address: 1907529740@qq.com.
⁴ Department of Medical Laboratory, The First Affiliated Hospital, Zhengzhou University, Zhengzhou, Henan Province, China. Electronic address: zdqyh09@126.com.
⁵ Department of Medical Laboratory, The First Affiliated Hospital, Zhengzhou University, Zhengzhou, Henan Province, China. Electronic address: yinhuiqing12@126.com.
⁶ Department of Medical Laboratory, The First Affiliated Hospital, Zhengzhou University, Zhengzhou, Henan Province, China. Electronic address: wanjh2009@163.com.
⁷ Department of Medical Laboratory, The First Affiliated Hospital, Zhengzhou University, Zhengzhou, Henan Province, China. Electronic address: hefucheng@zzu.edu.cn.

PMID: 32502839
DOI: 10.1016/j.biopha.2020.110313

Abstract

Accumulating evidence has demonstrated that long non-coding RNAs (lncRNAs) function as essential regulators in the development and progression of multiple tumors. However, the molecular mechanisms of MIR31HG in regulating ESCC progression remain unknown. Here, we confirmed that MIR31HG facilitated ESCC cells proliferation in vivo. Besides, MIR31HG knockdown increases the percentage of cells at the G1 phase, along with reduced arrest in S phase and MIR31HG overexpression exhibits the opposite effects. Overexpressed MIR31HG decreases the percentage of apoptotic ESCC cells. Interestingly, MIR31HG can function as a competing endogenous RNA by sponging miR-34a. The rescue experiments demonstrated that MIR31HG function is partially reversed by inhibiting miR-34a. In addition, we found c-Met is a target gene of miR-34a and is indirectly regulated by MIR31HG. Taken together, our findings revealed that MIR31HG promotes ESCC progression by regulating miR-34a/ c-Met axis and may provide a new prospective for exploration and understanding of the biological effects of esophageal squamous cell carcinoma.

Keywords: Esophageal squamous cell carcinoma; Long non-coding RNA; MIR31HG; MiR-34a; c-Met.

MeSH terms

Animals
Apoptosis
Cell Cycle Checkpoints
Cell Line, Tumor
Cell Proliferation
Esophageal Neoplasms / enzymology*
Esophageal Neoplasms / genetics
Esophageal Neoplasms / pathology
Esophageal Squamous Cell Carcinoma / enzymology*
Esophageal Squamous Cell Carcinoma / genetics
Esophageal Squamous Cell Carcinoma / pathology
Gene Expression Regulation, Neoplastic
Humans
Male
Mice, Inbred BALB C
Mice, Nude
MicroRNAs / genetics
MicroRNAs / metabolism*
Proto-Oncogene Proteins c-met / genetics
Proto-Oncogene Proteins c-met / metabolism*
RNA, Long Noncoding / genetics
RNA, Long Noncoding / metabolism*
Signal Transduction
Tumor Burden

Substances

MIRN34 microRNA, human
MicroRNAs
RNA, Long Noncoding
long noncoding RNA MIR31HG, human
MET protein, human
Proto-Oncogene Proteins c-met