Temporal dynamics of muscle optical properties during degeneration and regeneration in a canine muscle xenograft model

Michael E Nance; Mohammadreza Ravanfar; Mark Messler; Dongsheng Duan; Gang Yao

doi:10.1364/BOE.390936

Temporal dynamics of muscle optical properties during degeneration and regeneration in a canine muscle xenograft model

Biomed Opt Express. 2020 Apr 6;11(5):2383-2393. doi: 10.1364/BOE.390936. eCollection 2020 May 1.

Authors

Michael E Nance¹, Mohammadreza Ravanfar², Mark Messler¹, Dongsheng Duan^{1

2

3

4

5}, Gang Yao^{2

6}

Affiliations

¹ Department of Molecular Microbiology and Immunology, School of Medicine, University of Missouri, Columbia, MO 65201, USA.
² Department of Biomedical, Biological & Chemical Engineering, College of Engineering, University of Missouri, Columbia, MO 65201, USA.
³ Department of Neurology, School of Medicine, University of Missouri, Columbia, MO 65201, USA.
⁴ Department of Biomedical Sciences, College of Veterinary Medicine, University of Missouri, Columbia, MO 65201, USA.
⁵ DuanD@missouri.edu.
⁶ YaoG@missouri.edu.

Abstract

We studied time-dependent changes in muscle optical properties during degeneration and regeneration using polarization-sensitive optical coherence tomography (PSOCT). Excised canine muscle transplants in a xenograft mouse model were imaged ex vivo from 3- to 112-day post-transplantation. PSOCT images were quantified to evaluate post-transplantation changes of three optical/structural properties: attenuation, birefringence and fiber alignment. The birefringence and fiber alignment decreased after transplantation until 20∼30-day and recovered thereafter. The attenuation coefficient showed a reversed trend over the same period of time. These results suggest that optical properties could be used for monitoring skeletal muscle degeneration and regeneration.

Abstract

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