Effects of resveratrol on autophagy and the expression of inflammasomes in a placental trophoblast oxidative stress model

Abstract

Objective: We aim to investigate whether there is activation of NLRP1 and autophagy in trophoblast oxidative stress model. Resveratrol was taken to clarify its role in oxidative damage of placental trophoblasts.

Methods: H₂O₂ was added to HTR-8/SVneo cell for 3 h, then the ROS level and apoptosis panel was performed. The levels of IL-1β, caspase-1, NLRP1, LC3 and Beclin-1 were detected. Resveratrol was added after 8 h, the ROS level and apoptosis rate were detected, the expression of IL-1β, caspase-1, NLRP1, LC3 and Beclin-1 were detected.

Results: 300 μmol/L H₂O₂ for 3 h is the optimum combination in establishing the oxidative stress injury model (P < 0.01). LDH, ROS and MDA level was increased, the activity of SOD, CAT were declined (P < 0.01). Apoptosis rate increased (P < 0.01). The expression of IL-1β, caspase-1, NLRP1, LC3 and Beclin-1 protein was higher (P < .01). Resveratrol (50 μmol/L) treatment for 8 h could improve the changes caused by H₂O₂, increase the survival rate of cells (P < 0.01), reduce the release of LDH, decrease the level of MDA, increase the level of SOD and CAT (P < 0.01). The expression of IL-1β, caspase-1, NLRP1, LC3 and Beclin-1 protein decreased (P < 0.01).

Conclusion: Trophoblast oxidative damage model can be established under 300 μmol/L H₂O₂ for 3 h, the expression of NLRP1and autophagy after H₂O₂ treatment were detected. Resveratrol reduces apoptotic cells, thus ensuring the normal biological functions of trophoblasts.

Capsule: H₂O₂-induced oxidative stress damage model in HTR-8/SVneo cells can be successfully established under 300 μmol/L H₂O₂ for 3 h, resveratrol alleviates of H₂O₂-induced damage by its antioxidant and autophagy regulation function.

Keywords: Autophagy; HTR-8/SVneo; Inflammasome; Oxidative stress; Resveratrol.