Monodispersed mesoporous SiO2@metal-organic framework (MSN@MIL-101(Fe)) composites as sorbent for extraction and preconcentration of phytohormones prior to HPLC-DAD analysis

Peige Qin; Wenli Zhu; Lizhen Han; Xiaowan Zhang; Bing Zhao; Xuebin Zhang; Minghua Lu

doi:10.1007/s00604-020-04326-3

Monodispersed mesoporous SiO₂@metal-organic framework (MSN@MIL-101(Fe)) composites as sorbent for extraction and preconcentration of phytohormones prior to HPLC-DAD analysis

Mikrochim Acta. 2020 Jun 3;187(6):367. doi: 10.1007/s00604-020-04326-3.

Authors

Peige Qin¹, Wenli Zhu¹, Lizhen Han¹, Xiaowan Zhang¹, Bing Zhao², Xuebin Zhang², Minghua Lu³

Affiliations

¹ Henan International Joint Laboratory of Medicinal Plants Utilization, College of Chemistry and Chemical Engineering, Henan University, Kaifeng, 475004, Henan, China.
² Center for Multi-Omics Research, State Key Laboratory of Cotton Biology, Institute of Plant Stress Biology, Henan University, Kaifeng, 475004, Henan, China.
³ Henan International Joint Laboratory of Medicinal Plants Utilization, College of Chemistry and Chemical Engineering, Henan University, Kaifeng, 475004, Henan, China. mhlu@henu.edu.cn.

PMID: 32494885
DOI: 10.1007/s00604-020-04326-3

Abstract

The monodispersed mesoporous SiO₂@metal-organic framework (MSN@MIL-101(Fe)) composites were prepared by grafting MSN-NH₂ onto MIL-101(Fe) particles with a solvothermal method. The adsorption ability of the composites was greatly improved compared to that of pristine MSNs or MIL-101(Fe) for phytohormones (Phys). The MSN@MIL-101(Fe) composites were characterized by scanning electron microscopy, transmission electron microscopy, Fourier transform infrared spectroscopy, X-ray diffraction, energy dispersive spectrometer, and mapping analysis. Using MSN@MIL-101(Fe) composites as sorbent, a dispersive solid-phase extraction procedure (dSPE) was developed to extract three endogenous Phys (abscisic acid (ABA), indole-3-aceticacid (IAA), and indole-3-butyric acid (IBA)) and two exogenous Phys (1-naphthylacetic acid (1-NAA) and 2-naphthylacetic acid (2-NAA)) prior to HPLC-DAD analysis. The experimental parameters including sample volume, sorbent amount, adsorption time, adsorption pH, desorption time, and desorption solvent on extraction efficiency were optimized and evaluated. Under optimized conditions, the working range of 0.08 to 0.45 ng mL^-1 with enrichment factors from 144 to 207 were achieved. The linear range is 0.75-200 ng mL^-1 for IAA, 0.20-200 ng mL^-1 for ABA, and 1.0-200 ng mL^-1 for IBA, 1-NAA, and 2-NAA. With MSN@MIL-101(Fe) as sorbent for extraction of Phys and determination by HPLC-DAD, two endogenous Phys (IAA and ABA) were detected from mung bean sprouts which were made in a laboratory, and the results were further confirmed by high-resolution mass spectrometry. The composites can be applied to extract other small molecules, which have similar chemical structures with Phys in biological, environmental, and food samples. Graphical abstract Schematic presentation of a dispersive solid-phase extraction using monodispersed mesoporous SiO₂@metal-organic framework composites (MSNs@MIL-101(Fe)) as the sorbent for extraction, clean-up, and preconcentration of phytohormones in mung bean sprouts prior to HPLC-DAD analysis.

Keywords: Abscisic acid (ABA); Dispersive solid-phase extraction (dSPE); Indole-3-aceticacid (IAA); Indole-3-butyric acid (IBA); Mass spectrometry (MS); Metal-organic framework (MOF); Mung bean sprouts; Naphthylacetic acid (NAA); Plant hormones; Sample pretreatment.

Publication types

Research Support, Non-U.S. Gov't

Abstract

Publication types

Grants and funding