Background/aim: To determine the effect of different doses of capsaicin on AgNOR protein synthesis in human colon adenocarcinoma derivate from colon cancer (Caco-2 cell).
Materials and methods: In this experimental study, after the cultured of Caco-2 cell line, the cells are divided into 4 groups as control and different capsaicin exposed doses (25uμ, 50uμ, and 75uμ). Mean AgNOR number and total AgNOR area/nuclear area (TAA/NA) were calculated.
Results: A significant differences were detected between control and capsaicin (50uμ) (P = 0.001), between control and capsaicin (75uμ) (P = 0.000), between capsaicin (25uμ) and capsaicin (50uμ) (P = 0.001) and between capsaicin (25uμ) and capsaicin (75uμ) (P = 0.000) for TAA/NA. Also, there were significant differences between control and capsaicin (50uμ) (P = 0.001), between control and capsaicin (75uμ) (P = 0.000), between capsaicin (25uμ) and capsaicin (50uμ) (P = 0.000) and between capsaicin (25uμ) and capsaicin (75uμ) (P = 0.000) for mean AgNOR number.
Conclusion: A certain amount of capsaicin has a protective effect against colon adenocarcinoma and the dose concentrations are important for the most reliable treatment.
Keywords: AgNOR; caco-2 cell line; capsaicin; human colon adenocarcinoma; rDNA.
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