Clinical bacteriological aspects of the Human Amniotic Membrane in the diabetic foot. A case report

Laura Maria Chisari; Grasso Antonino; Giuseppe Chisari; Antonio Maria Borzì; Clara Grazia Chisari

doi:10.1016/j.amsu.2020.04.020

Clinical bacteriological aspects of the Human Amniotic Membrane in the diabetic foot. A case report

Ann Med Surg (Lond). 2020 May 16:55:174-176. doi: 10.1016/j.amsu.2020.04.020. eCollection 2020 Jul.

Authors

Laura Maria Chisari¹, Grasso Antonino², Giuseppe Chisari¹, Antonio Maria Borzì³, Clara Grazia Chisari⁴

Affiliations

¹ Department of Biomedical and Biotechnological Sciences, University of Catania, Italy.
² Department of General Surgery and Medical Surgical Specialties, University of Catania, Italy.
³ Research Center "The Great Senescence", University of Catania, Italy.
⁴ Department of Medical-Surgical Sciences and Advanced Technologies "G.F. Ingrassia", University of Catania, Italy.

Abstract

The phases of a cutaneous infection are initially the adhesion of the microorganism to the cells of the host, followed by the invasion of the tissues, than the elaboration of toxins and the escape from the defense systems of the host. The Human Amniotic Membrane (HAM) is extracted from the placenta of donors after caesarean section. The amnios is characterized by a monolayer of epithelial cells, a basement membrane and an avascular stroma of collagen. The HAM showed to promote chronic wound healing. We evaluated the "in vivo" and "in vitro" activity and efficacy of the HAM in subjects with chronic diabetic skin lesion. This clinical case showed that the HAM promote the wound healing of complex chronic skin lesion in a follow-up period of 6 months after the first graft.

Keywords: Aerobic bacteria; Anaerobic bacteria; Diabetic patient; Human amniotic membrane; Skin ecosystem; Skin lesions.

Publication types

Case Reports