Prevalence and antibiotic resistance patterns of Vibrio parahaemolyticus isolated from different types of seafood in Selangor, Malaysia

Chia Wanq Tan; Yaya Rukayadi; Hanan Hasan; Tze Young Thung; Epeng Lee; Wendy Dayang Rollon; Hirofumi Hara; Ahmad Yaman Kayali; Mitsuaki Nishibuchi; Son Radu

doi:10.1016/j.sjbs.2020.01.002

Prevalence and antibiotic resistance patterns of Vibrio parahaemolyticus isolated from different types of seafood in Selangor, Malaysia

Saudi J Biol Sci. 2020 Jun;27(6):1602-1608. doi: 10.1016/j.sjbs.2020.01.002. Epub 2020 Jan 16.

Authors

Affiliations

¹ Department of Food Science, Faculty of Food Science and Technology, Universiti Putra Malaysia, UPM Serdang, 43400 Selangor Darul Ehsan, Malaysia.
² Food Safety and Food Integrity, Institute of Tropical Agriculture and Food Security (ITAFoS), Universiti Putra Malaysia, UPM Serdang, 43400 Selangor Darul Ehsan, Malaysia.
³ Department of Environmental Engineering and Green Technology, Malaysia-Japan International Institute of Technology, Universiti Teknologi Malaysia, Kuala Lumpur 54100, Malaysia.
⁴ Center for Southeast Asian Studies, Kyoto University, Kyoto 606-8501, Japan.

Abstract

Vibrio parahaemolyticus is a foodborne bacterial pathogen that may cause gastroenteritis in humans through the consumption of seafood contaminated with this microorganism. The emergence of antimicrobial and multidrug-resistant bacteria is another serious public health threat worldwide. In this study, the prevalence and antibiotic susceptibility test of V. parahaemolyticus in blood clams, shrimps, surf clams, and squids were determined. The overall prevalence of V. parahaemolyticus in seafood was 85.71% (120/140), consisting of 91.43% (32/35) in blood clam, 88.57% (31/35) in shrimps, 82.86% (29/35) in surf clams, and 80% (28/35) in squids. The majority of V. parahaemolyticus isolates from the seafood samples were found to be susceptible to most antibiotics except ampicillin, cefazolin, and penicillin. The MAR indices of V. parahaemolyticus isolates ranged from 0.04 to 0.71 and about 90.83% of isolates were found resistant to more than one antibiotic. The high prevalence of V. parahaemolyticus in seafood and multidrug-resistant isolates detected in this study could pose a potential risk to human health and hence appropriate control methods should be in place to minimize the potential contamination and prevent the emergence of antibiotic resistance.

Keywords: Antibiogram; MAR; Multidrug-resistant; Seafood; Vibrio.