NanoTiO2 materials mitigate mercury uptake and effects on green alga Chlamydomonas reinhardtii in mixture exposure

Abstract

The present study examined the effect of titanium dioxide nanoparticles (nanoTiO₂) and mercury (Hg) compounds on the green alga, Chlamydomonas reinhardtii. Mixtures containing nanoTiO₂ of different primary sizes (5 nm, 15 nm and 20 nm), inorganic Hg (IHg) or monomethyl Hg (CH₃Hg⁺, MeHg) were studied and compared with individual treatments. Oxidative stress and membrane damage were examined. Stability of nanoTiO₂ materials in terms of hydrodynamic size and surface charge as well as Hg adsorption on different nanoTiO₂ materials were characterized. The uptake of Hg compounds in the absence and presence of nanoTiO₂ was also quantified. Results show that increasing concentrations of nanoTiO₂ with different primary size diminished oxidative stress and membrane damage induced by high concentrations of IHg or MeHg, due to the adsorption of Hg on the nanoTiO₂ aggregates and consequent decrease of cellular Hg concentrations. The observed alleviation effect of nanoTiO₂ materials on Hg biouptake and toxicity was more pronounced for the materials with smaller primary size. IHg adsorbed onto the nanoTiO₂ materials to a higher extent than MeHg. The present study highlights that the effects of contaminants are modulated by the co-existing engineered nanomaterials; therefore, it is essential to get a better understanding of their combined effect in the environment.

Keywords: bioavailability; cocktail effect; mercury; methylmercury; mixtures; nanoTiO(2); phytoplankton; toxicity.