Abstract
Label-based functional studies of biomolecules in their native environment require labeling reactions inside living cells. In cell spin labeling using alkyne-azide click chemistry with a Gd3+-DOTAM-azide complex is shown to provide high spin label stability and narrow EPR lines for EPR spectroscopic detection of a spin labeled protein in living cells at ambient temperatures.
MeSH terms
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Acetamides / chemistry
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Alkynes / chemistry
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Azides / chemistry
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Click Chemistry
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Electron Spin Resonance Spectroscopy
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Escherichia coli / chemistry*
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Escherichia coli / cytology
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Gadolinium / chemistry*
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Green Fluorescent Proteins / analysis*
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Heterocyclic Compounds, 1-Ring / chemistry
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Molecular Structure
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Spin Labels*
Substances
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1,4,7,10-tetrakis(carbamoylmethyl)-1,4,7,10-tetraazacyclododecane
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Acetamides
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Alkynes
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Azides
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Heterocyclic Compounds, 1-Ring
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Spin Labels
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enhanced green fluorescent protein
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Green Fluorescent Proteins
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Gadolinium