Investigation of the interaction between lighting and mixing applied during the photo-fermentation biohydrogen production process from agricultural waste

Abstract

To better clarify the effect of lighting and mixing on the hydrogen production from agricultural waste, experiments under diverse constant and dynamic light intensities and mixing speeds were conducted. Cumulative hydrogen yield, hydrogen production rate, OD₆₆₀, pH, reducing sugar concentration were monitored. Results showed that mixing had positive effects on high substrate concentration. The interaction between lighting and mixing was drawn. Higher light intensity (7000 Lux) was suitable for hydrogen production under mixing. Higher light intensity with higher mixing speed (150 RPM) showed the highest cumulative hydrogen yield of 78.1 mL/g TS. Different periods of hydrogen production process required distinct lighting and mixing. Combined with Gompertz model, dynamic lighting and mixing strategies were discussed. Dynamic light intensity (4000-7000-4000 Lux) accompanied with dynamic mixing speed (50-150-50 RPM) was the optimal condition for PFHP. Highest hydrogen yield of 84.7 mL/g TS and highest light conversion efficiency of 36.32% were obtained.

Keywords: Dynamic control; Hydrogen production performance; Light conversion efficiency; Light intensity; Mixing speed.