A comprehensive study of lipid profiles of round scad (Decapterus maruadsi) based on lipidomic with UPLC-Q-Exactive Orbitrap-MS

Chen He; Zexin Sun; Xingchen Qu; Jun Cao; Xuanri Shen; Chuan Li

doi:10.1016/j.foodres.2020.109138

A comprehensive study of lipid profiles of round scad (Decapterus maruadsi) based on lipidomic with UPLC-Q-Exactive Orbitrap-MS

Food Res Int. 2020 Jul:133:109138. doi: 10.1016/j.foodres.2020.109138. Epub 2020 Mar 2.

Authors

Chen He¹, Zexin Sun¹, Xingchen Qu¹, Jun Cao², Xuanri Shen¹, Chuan Li³

Affiliations

¹ Hainan Provincial Engineering Research Centre of Aquatic Resources Efficient Utilization in the South China Sea, College of Food Science and Engineering, Hainan University, Haikou 570228, China.
² Hainan Provincial Engineering Research Centre of Aquatic Resources Efficient Utilization in the South China Sea, College of Food Science and Engineering, Hainan University, Haikou 570228, China. Electronic address: juncaoyd2007@126.com.
³ Hainan Provincial Engineering Research Centre of Aquatic Resources Efficient Utilization in the South China Sea, College of Food Science and Engineering, Hainan University, Haikou 570228, China; Collaborative Innovation Center of Seafood Deep Processing, Dalian Polytechnic University, Dalian 116034, China. Electronic address: lichuanbest@126.com.

PMID: 32466915
DOI: 10.1016/j.foodres.2020.109138

Abstract

Round scad (Decapterus maruadsi) is rich in eicosapentaenoic acid (EPA) and docosahexaenoic acid (DHA). However, there is no comprehensive information covering its lipidomic profile. In this study, lipid profiles including fatty acid composition, distribution and detailed structure information were explored using gas chromatography-flame ionization detector (GC-FID) and ultra-high-performance liquid chromatography coupled with hybrid quadrupole-orbitrap mass spectrometry (UPLC-Q-Exactive Orbitrap-MS). The results showed that triacylglycerol (TG) was the dominant class, and the EPA and DHA were concentrated as glycerophospholipid (GP). The saturated fatty acids and monounsaturated fatty acids were mainly esterified in positions 1 and 3 of TG and sn-1-position of GP, while the polyunsaturated fatty acids (PUFA) were mainly esterified in sn-2-position of TG and GP. A total of 1282 species from six classes (21 subclasses) including glycerolipids (GL), sphingolipids (SP), GP, fatty acyls (FA), saccharolipids (SL) and prenol lipids (PR) were identified. Several molecular species were characterized with PUFA, especially EPA and DHA in GP. Considering the superior fatty acids composition and distribution of round scad, it is deserved for further exploitation of its marine lipid source on account of the healthy and nutritional functions.

Keywords: DHA; EPA; Lipid profiles; Lipidomics; Round scad (Decapterus maruadsi); Stereospecific analysis; UPLC-Q-Exactive Orbitrap-MS.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Animals
Chromatography, High Pressure Liquid
Fatty Acids*
Gas Chromatography-Mass Spectrometry
Lipidomics*
Mass Spectrometry

Substances

Fatty Acids