Evaluation of the level of selected iron-related proteins/receptors in the liver of rats during separate/combined vanadium and magnesium administration

Agnieszka Ścibior; Iwona Hus; Joanna Mańko; Dariusz Jawniak

doi:10.1016/j.jtemb.2020.126550

Evaluation of the level of selected iron-related proteins/receptors in the liver of rats during separate/combined vanadium and magnesium administration

J Trace Elem Med Biol. 2020 May 15:61:126550. doi: 10.1016/j.jtemb.2020.126550. Online ahead of print.

Authors

Agnieszka Ścibior¹, Iwona Hus², Joanna Mańko³, Dariusz Jawniak⁴

Affiliations

¹ Laboratory of Oxidative Stress, Centre for Interdisciplinary Research, The John Paul II Catholic University of Lublin, Poland. Electronic address: cellbiol@kul.lublin.pl.
² Institute of Hematology and Transfusion Medicine, Warsaw, Poland. Electronic address: iwonach.hus@gmail.com.
³ Clinic of Haematooncology and Bone Marrow Transplantation, Medical University, Lublin, Poland. Electronic address: joanna_m@go2.pl.
⁴ Clinic of Haematooncology and Bone Marrow Transplantation, Medical University, Lublin, Poland. Electronic address: dkjk@wp.pl.

PMID: 32464446
DOI: 10.1016/j.jtemb.2020.126550

Abstract

Background: The current knowledge about the effects of vanadium (V) on iron (Fe)-related proteins and Fe homeostasis (which is regulated at the systemic, organelle, and cellular levels) is still insufficient.

Objective: This fact and our earlier results prompted us to conduct studies with the aim to explain the mechanism of anemia accompanied by a rise in hepatic and splenic Fe deposition in rats receiving sodium metavanadate (SMV) separately and in combination with magnesium sulfate (MS).

Results: We demonstrated for the first time that SMV (0.125 mg V/mL) administered to rats individually and in conjunction with MS (0.06 mg Mg/mL) for 12 weeks did not cause significant differences in the hepatic hepcidin (Hepc) and hemojuvelin (HJV) concentrations, compared to the control. In comparison with the control, there were no significant changes in the concentration of transferrin receptor 1 (TfR1) in the liver of rats treated with SMV and MS alone (in both cases only a downward trend of 14% and 15% was observed). However, a significant reduction in the hepatic TfR1 level was found in rats receiving SMV and MS simultaneously. In turn, the concentration of transferrin receptor 2 (TfR2) showed an increasing trend in the liver of rats treated with SMV and/or MS.

Conclusions: The experimental data suggest that the pathomechanism of the SMV-induced anemia is not associated with the effect of V on the concentration of Hepc in the liver, as confirmed by the unaltered hepatic HJV and TfR1 levels. Therefore, further studies are needed in order to check whether anemia that developed in the rats at the SMV administration (a) results from the inhibitory effect of V on erythropoietin (EPO) production, (b) is related to the effect of V on the induction of matriptase-2 (TMPRSS6) expression, or (c) is associated with the influence of this metal on haem synthesis.

Keywords: Anemia; Interactions; Iron-binding proteins; Iron-regulatory proteins; Magnesium; Vanadium.