Photothermal modulation of human stem cells using light-responsive 2D nanomaterials

Proc Natl Acad Sci U S A. 2020 Jun 16;117(24):13329-13338. doi: 10.1073/pnas.1914345117. Epub 2020 May 27.

Abstract

Two-dimensional (2D) molybdenum disulfide (MoS2) nanomaterials are an emerging class of biomaterials that are photoresponsive at near-infrared wavelengths (NIR). Here, we demonstrate the ability of 2D MoS2 to modulate cellular functions of human stem cells through photothermal mechanisms. The interaction of MoS2 and NIR stimulation of MoS2 with human stem cells is investigated using whole-transcriptome sequencing (RNA-seq). Global gene expression profile of stem cells reveals significant influence of MoS2 and NIR stimulation of MoS2 on integrins, cellular migration, and wound healing. The combination of MoS2 and NIR light may provide new approaches to regulate and direct these cellular functions for the purposes of regenerative medicine as well as cancer therapy.

Keywords: cell adhesion; cell–nanoparticles interactions; human mesenchymal stem cells; two-dimensional (2D) nanomaterials; whole-transcriptome sequencing.

Publication types

  • Research Support, N.I.H., Extramural

MeSH terms

  • Cell Adhesion / radiation effects
  • Cell Movement / radiation effects
  • Cell Survival
  • Disulfides / chemistry
  • Disulfides / metabolism
  • Disulfides / radiation effects*
  • Gene Expression Profiling
  • Humans
  • Infrared Rays
  • Integrins / genetics
  • Integrins / metabolism
  • Mesenchymal Stem Cells / cytology
  • Mesenchymal Stem Cells / metabolism
  • Mesenchymal Stem Cells / radiation effects*
  • Molybdenum / chemistry
  • Molybdenum / metabolism
  • Molybdenum / radiation effects*
  • Nanostructures / chemistry
  • Nanostructures / radiation effects*
  • Photosensitizing Agents
  • Signal Transduction / radiation effects

Substances

  • Disulfides
  • Integrins
  • Photosensitizing Agents
  • Molybdenum
  • molybdenum disulfide